Circular RNA profile of breast cancer brain metastasis: identification of potential biomarkers and therapeutic targets

To explore the circular RNA (circRNA) profile of breast cancer brain metastasis (BCBM). RNA-seq was performed to identify the circRNA expression profile of brain metastatic breast cancer cell line 231-BR, in comparison with its parental nonspecific metastatic cell line MDA-MB-231. A total of 215 upr...

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Bibliographic Details
Published in:Epigenomics 2018-12, Vol.10 (12), p.1619-1630
Main Authors: Fu, Bo, Zhang, Anqi, Li, Mingli, Pan, Li, Tang, Wenqiang, An, Meng, Liu, Wei, Zhang, Jianqiong
Format: Article
Language:English
Subjects:
biomarker
Biomarkers
Brain
Brain cancer
Breast cancer
breast cancer brain metastasis
Cancer
Circular RNA
Deoxyribonucleic acid
DNA
Electrophoresis
Encyclopedias
expression profile
Gel electrophoresis
Gene expression
Genomes
Identification
Identification methods
Liver cancer
Metastases
Metastasis
MicroRNAs
miRNA
Ontology
Proteins
Ribonucleic acid
RNA
Therapeutic applications
therapeutic target
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Summary:To explore the circular RNA (circRNA) profile of breast cancer brain metastasis (BCBM). RNA-seq was performed to identify the circRNA expression profile of brain metastatic breast cancer cell line 231-BR, in comparison with its parental nonspecific metastatic cell line MDA-MB-231. A total of 215 upregulated and 191 downregulated circRNAs were identified. The expression levels of ten randomly selected majorly altered circRNAs were re-examined by real-time quantitative PCR and agarose gel electrophoresis, and the following alterations were verified: upregulation of hsa_circ_0001944, hsa_circ_0001481, hsa_circ_0000646, hsa_circ_0001006 and hsa_circ_0000732, and downregulation of hsa_circ_0001910, hsa_circ_0008285 and hsa_circ_0000002. CircRNA/miRNA analysis revealed that hsa_circ_0001944 may be involved in BCBM through sponging up miR-509 and interfering with its binding to the downstream targets. This study provides a leading and fundamental circRNA profile of BCBM.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2018-0090