Safety, functional properties and technological performance in whey-based media of probiotic candidates from human breast milk

• Published in: International microbiology 2019-06, Vol.22 (2), p.265-277
• Main Authors: Zacarías, María Florencia, Binetti, Ana, Bockelmann, Wilhelm, Reinheimer, Jorge, Heller, Knut, Vinderola, Gabriel
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Applied Microbiology; Bifidobacterium - drug effects; Bifidobacterium - growth & development; Bifidobacterium - isolation & purification; Bifidobacterium - metabolism; Bile Acids and Salts - toxicity; Biomedical and Life Sciences; Breast milk; Breastfeeding & lactation; Chloramphenicol; Chloromycetin; Clindamycin; Culture Media - chemistry; Digestion; Erythromycin; Eukaryotic Microbiology; Female; Freeze drying; Gastric Acid - metabolism; Humans; Hydrophobicity; Lactobacilli; Lactobacillus - drug effects; Lactobacillus - growth & development; Lactobacillus - isolation & purification; Lactobacillus - metabolism; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Sensitivity Tests; Microbial Viability - drug effects; Microbiology; Microorganisms; Milk; Milk, Human - microbiology; Original Article; Pancreatin; Pancreatin - toxicity; Postpartum; Probiotics; Probiotics - adverse effects; Probiotics - isolation & purification; Storage conditions; Strains (organisms); Streptomycin; Vancomycin; Whey; Whey - metabolism
• ISSN: 1139-6709; 1618-1905
• DOI: 10.1007/s10123-018-00046-0

We aimed at isolating and characterising microorganisms present in human breast milk with probiotic potential. In an 8-week postpartum sampling period, two strains of bifidobacteria ( Bifidobacterium longum LM7a and Bifidobacterium dentium LM8a’) and four strains of lactobacilli were isolated, all during the first 4-week postpartum. B. longum LM7a and B. dentium LM8a’, together with four strains previously isolated from breast milk ( Bifidobacterium lactis INL1, INL2, INL4 and INL5), were considered for further studies. Susceptibility of the strains to tetracycline, erythromycin, clindamycin, streptomycin, vancomycin and chloramphenicol was evaluated and the isolates exhibited, in general, the same properties as previously reported for bifidobacteria. All isolates showed low hydrophobicity and B. lactis and B. longum strains had satisfactory resistance to gastric digestion and bile shock, but not to pancreatin. B. lactis INL1, B. longum LM7a and B. dentium LM8a’ were selected for some comparative technological studies. In particular, B. lactis INL1 displayed technological potential, with satisfactory growth in cheese whey-based media in biofermentor and resistance to freeze-drying, accelerated storage conditions and simulated gastric digestion.