The influence of serum‐supplemented culture media in a transwell migration assay

Our work cautions against the use of serum‐supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FB...

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Bibliographic Details
Published in:Cell biology international 2019-10, Vol.43 (10), p.1201-1204
Main Authors: Omar Zaki, Siti Sarah, Kanesan, Livashini, Leong, Ming Yeh Danielle, Vidyadaran, Sharmili
Format: Article
Language:English
Subjects:
Animals
BV2 microglia
Cell Culture Techniques - methods
Cell Movement
Cells, Cultured
chemoattractant
Chemotactic factors
Chemotactic Factors - chemistry
Culture media
Culture Media - chemistry
Microglia
Microglia - cytology
serum
Serum - chemistry
transwell migration assay
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Summary:Our work cautions against the use of serum‐supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS‐supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.11122