Relationship between ureidic/amidic metabolism and antioxidant enzymatic activities in legume seedlings

• Published in: Plant physiology and biochemistry 2019-05, Vol.138, p.1-8
• Main Authors: Quiles, Francisco A., Galvez-Valdivieso, Gregorio, Guerrero-Casado, Jose, Pineda, Manuel, Piedras, Pedro
• Format: Article
• Language: English
• Subjects: Allantoinase; Antioxidant activities; Legumes; Seedlings; Ureide
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2019.02.016

Ureides are nitrogenous compounds with a special function in some legume under nitrogen fixing conditions, the ureidic legumes. In this group, ureides are the predominant nitrogen transport molecule from nodules to the upper part, whereas amidic legumes use amides as nitrogen transport compounds. In this study, the ureide levels have been analysed in seedlings from four ureidic and four amidic legume plants. It has been found that the differentiation among ureide and amide plants already exists in seedlings during early seedling development, with high levels of ureide and allantoinase activity in cotyledons and embryonic axes from ureide plants. Since ureides have been implicated in the response of plant to several stress, total hydrosoluble antioxidant capacity and the levels of several antioxidant activities have been determined and compared among these two legume groups. The total antioxidant capacity did not follow any differential pattern in cotyledons or embryonic axes for the analysed plants. The levels of superoxide dismutase, guaiacol peroxidase and ascorbate peroxidase in both embryonic axes and cotyledons are statistical different between amide and ureide seedlings, whereas the catalase activity was similar among these groups of plants. We discuss than amides and ureides could follow different strategies to protect against oxidation. •The concentration of ureides is higher in seedlings from ureidic than amidic legumes.•The total antioxidant capacity in all the seedlings are similar.•Several antioxidant activities show a different pattern in embryonic axes from ureidic and amidic legumes.