Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: A prognosticative approach for “Royal disease”

• Published in: Biosensors & bioelectronics 2019-04, Vol.131, p.128-135
• Main Authors: Letchumanan, Iswary, Gopinath, Subash C.B., Md Arshad, M.K., Anbu, Periasamy, Lakshmipriya, Thangavel
• Format: Article
• Language: English
• Subjects: Amperometry; Aptamer; ELASA; Factor IX; Gold nano-urchin; Royal disease
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2019.02.006

This article is clearly presenting the development of a biosensor for human factor IX (FIX) to diagnose the blood clotting deficiency, a so-called ‘Royal disease’ using an interdigitated electrode (IDE) with the zinc oxide surface modification. Gold nano-urchins (GNUs) with 60 nm in diameter was integrated into a streptavidin-biotinylated aptamer strategy to enhance the active surface area. Two different comparative studies have been done to validate the system to be practiced in the current work holds with a higher capability for the high-performance sense. Whereby, the presence and absence of GNUs in the aptasensing system for FIX interaction were investigated using the amperometric measurement, using a linear sweep voltage of 0–2 V at 0.01 V step voltage. The detection limit was 6 pM based on 3σ calculation when GNUs integrated aptamer assay was utilized for FIX detection, which shows 8 folds sensitivity enhancement comparing the condition in the absence of GNU and 50 folds higher than sensitive radio-isotope and surface plasmon resonance assays. Albeit, the surface and molecular characterizations were well demonstrated by scanning electron microscopy, atomic force microscopy, 3D nano-profilometry and further supports were rendered by UV–Vis spectroscopy and Enzyme-linked apta-sorbent assay (ELASA). Furthermore, the spiking experiment was done by FIX-spikes in human blood serum in order to demonstrate the stability with a higher non-fouling. [Display omitted] •Gold nano-urchins (60 nm) were integrated into an aptamer-based sensing solution.•The LOD for Factor IX was 6 pM with the GNUs integrated aptamer assay system.•8 folds enhancement in LOD with GNU. Supported by Enzyme-linked Apta-sorbent assay.•GNU & molecular analysis were supported by morphological and optical analyses.•Factor IX-spiked in blood serum to demonstrate the stability.