Characterization and in vitro evaluation of chitosan/konjac glucomannan bilayer film as a wound dressing

• Published in: Carbohydrate polymers 2019-05, Vol.212, p.59-66
• Main Authors: Gomes Neto, Reginaldo Jose, Genevro, Giovana Maria, Paulo, Letícia de Almeida, Lopes, Patrícia Santos, de Moraes, Mariana Agostini, Beppu, Marisa Masumi
• Format: Article
• Language: English
• Subjects: Bandages; Bilayer film; Biomaterials; Biopolymers; Blend film; Candida albicans - drug effects; Candida albicans - physiology; Casting; Chitosan - chemical synthesis; Chitosan - pharmacology; Chromatography, Gel - methods; Drug Evaluation, Preclinical - methods; Escherichia coli - drug effects; Escherichia coli - physiology; Mannans - chemical synthesis; Mannans - pharmacology; Plants; Tensile Strength - drug effects; Tensile Strength - physiology; X-Ray Diffraction - methods
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.02.017

[Display omitted] •A two-step casting technique was used to prepare KGM/CHI bilayer films.•The bilayer films preserved the physicochemical properties of each polymer.•The two polymers presented good thermostability and miscibility.•The bilayer films exhibit low cytotoxicity and inhibit microbial penetration.•The bilayer films created a suitable moist environment for wound healing. A novel bilayer film of chitosan and konjac glucomannan were prepared by the two-step casting technique. Blend films were also prepared to investigate the interactions between the two polymers in the interfacial region of the bilayer structure. Scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis showed that, unlike in the blends, the physicochemical properties of each biopolymer were preserved in the bilayer film. Differential scanning calorimetry and thermogravimetric analysis also indicated a good thermostability and miscibility for both polymers, probably due to strong hydrogen bonds between their polymer chains. Biological, mechanical and water vapor transmission tests showed a high biocompatibility, low cytotoxicity, and suitable mechanical and barrier properties of the bilayer films for wound dressing applications.