Synergism of Pseudomonas aeruginosa (LSE-2) nodule endophyte with Bradyrhizobium sp. (LSBR-3) for improving plant growth, nutrient acquisition and soil health in soybean

• Published in: World journal of microbiology & biotechnology 2019-03, Vol.35 (3), p.47-17, Article 47
• Main Authors: Kumawat, K. C., Sharma, Poonam, Sirari, Asmita, Singh, Inderjeet, Gill, B. S., Singh, Ummed, Saharan, Krishna
• Format: Article
• Language: English
• Subjects: Acetic acid; Applied Microbiology; Biochemistry; Biofertilizers; Biofilms; Biofilms - growth & development; Biomedical and Life Sciences; Biotechnology; Bradyrhizobium; Bradyrhizobium - isolation & purification; Bradyrhizobium - physiology; Cell walls; Consortia; Crop yield; Endophytes; Environmental Engineering/Biotechnology; Flowering; Glycine max; Glycine max - growth & development; Glycine max - microbiology; India; Indoleacetic acid; Indoleacetic Acids - metabolism; Inoculation; Life Sciences; Microbiology; Nitrogen - analysis; Nutrients; Original Paper; Oxidoreductases - analysis; Parameters; Pathogenicity; Pathogens; Phosphates - metabolism; Phosphoric Monoester Hydrolases - analysis; Phylogeny; Plant Development; Plant growth; Plant Roots - chemistry; Plant Roots - microbiology; Plant Shoots - chemistry; Plant Shoots - microbiology; Potassium - analysis; Pseudomonas aeruginosa; Pseudomonas aeruginosa - classification; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - isolation & purification; Pseudomonas aeruginosa - physiology; RNA, Ribosomal, 16S - genetics; Siderophores - metabolism; Soil - chemistry; Soil Microbiology; Soil quality; Solubility; Solubilization; Soybeans; Symbiosis; Synergism; Synergistic effect; Virulence; Zinc; Zinc - metabolism
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-019-2622-0

The present study was aimed to assess the scope of native potential endophyte Pseudomonas aeruginosa (LSE-2) strain (KX925973) with recommended Bradyrhizobium sp. (LSBR-3) (KF906140) for synergistic effect to develop as consortium biofertilizer of soybean. A total of 28 non-rhizobial endophytic bacteria were isolated from cultivated and wild sp. of soybean. All isolates were screened for multifarious PGP traits viz . Indole-3-acetic acid (IAA), phosphate (P) and zinc (Zn) solubilization, siderophore, cell wall degrading enzymes and pathogenicity. Compatible of LSBR-3 and LSE-2 enhanced IAA, P-solubilization, 1-aminocyclopropane-carboxylate deaminase and biofilm formation over the single inoculant treatment. Further, consortium was evaluated in vivo for growth, symbiotic traits, nutrient acquisition, soil quality parameters and yield attributes of soybean. Improvement in growth parameters were recorded with dual inoculant LSBR-3 + LSE-2 as compared to LSBR-3 alone and un-inoculated control treatments. Significantly (p ≥ 0.05) high symbiotic and soil quality parameters (phosphatase and soil dehydrogenase activity) was recorded with LSBR-3 + LSE-2 at vegetative and flowering stage as compared to LSBR-3 alone and un-inoculated control treatments. Single inoculation of LSBR-3 improved grain yield by 4.25% over the un-inoculated control treatment, further, enhancement in yield was recorded with consortium inoculant (LSBR-3 and LSE-2) by 3.47% over the LSBR-3 alone. Application of consortium inoculant (LSBR-3 + LSE-2) gave an additional income of Rs. 5089/ha over the un-inoculated control treatment. The results, thus strongly suggest that endophytic diazotroph LSE-2 can be used as potent bio-inoculant along with LSBR-3 as bio-enhancer for improving soybean productivity in a sustainable system.