Retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) restore the germline competence of in vitro cultured chicken blastodermal cells

Chicken blastodermal cells (BCs) are pluripotent stem cells derived from early embryos and may be easily obtained and manipulated. However, in vitro cultured BCs have extremely low germline capacity, which may limit their applications. Research on the germ cell differentiation of mammalian pluripote...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2019-03, Vol.55 (3), p.169-176
Main Authors: Tang, Xiaochuan, Shi, Jun, Qin, Xiaolian, Xiao, Ning, Li, Rongyang, Hu, Hao, Yang, Fengshuo, Shi, Deshun, Wang, Xiaoli
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
BIOTECHNOLOGY
Bone morphogenetic protein 4
Cell Biology
Cell Culture
Cell differentiation
Chickens
Cultured meat
Developmental Biology
Differentiation (biology)
Embryos
Gene expression
Life Sciences
Organic chemistry
Pluripotency
Poultry
Proteins
Retinoic acid
Stem Cells
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Summary:Chicken blastodermal cells (BCs) are pluripotent stem cells derived from early embryos and may be easily obtained and manipulated. However, in vitro cultured BCs have extremely low germline capacity, which may limit their applications. Research on the germ cell differentiation of mammalian pluripotent cells using chemical-inducing agents has gained popularity, and tremendous achievements have been made. Whether chemical-inducing agents allow acquirement of germline competence in BCs is, however, questionable. In this study, retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted the expression of germline-specific genes and restored the germline competence of in vitro cultured BCs. Moreover, BCs induced with RA and BMP4 could efficiently produce gonadal chimeric chick embryos. These results may greatly enhance the potential applications of BCs in biotechnology and basic research.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-019-00324-9