Revealing mcr-1-positive ESBL-producing Escherichia coli strains among Enterobacteriaceae from food-producing animals (bovine, swine and poultry) and meat (bovine and swine), Portugal, 2010–2015

• Published in: International journal of food microbiology 2019-05, Vol.296, p.37-42
• Main Authors: Clemente, Lurdes, Manageiro, Vera, Correia, Ivone, Amaro, Ana, Albuquerque, Teresa, Themudo, Patrícia, Ferreira, Eugénia, Caniça, Manuela
• Format: Article
• Language: English
• Subjects: Animal feed; Animals; Bacteria; Colistin; E coli; Enterobacteriaceae; ESBL; Escherichia coli; Food; Food production; Food-producing animals; Livestock; mcr-1 emergence; MCR-1.9; Meat; Meat products; PMAβ; Salmonella; Swine
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2019.02.006

We screened 1840 Enterobacteriaceae isolates from food-producing animals, meat, meat products and animal feed, for the detection of plasmid-mediated colistin resistance, during 2010–2015. The mcr-1 gene was detected in 8.0% (97/1206) Escherichia coli and in 0.47% (3/634) Salmonella enterica isolates, with a high number of mcr-1 positive E. coli isolates (45.7%) being extended-spectrum β-lactamase or plasmid-mediated AmpC β-lactamase co-producers. No mcr-2 gene was detected. Our findings highlight the spread of mcr-1 genes within a wide-ranging sample of food-producing animals and meat, in Portugal. •Spread of MCR-1 within a wide-ranging sample of food-producing animals and meat•Occurrence of mcr genes in E. coli isolates from turkeys, swine and broilers•High number of mcr positive E. coli isolates that were ESBL or PMAβ co-producers•First description of MCR-1-producing Salmonella Reading from bovine meat•New mcr-1.9 variant reported in an Escherichia coli from a swine