Effect of short-term sterigmatocystin exposure on lipid peroxidation and glutathione redox system and expression of glutathione redox system regulatory genes in common carp liver

• Published in: Toxicon (Oxford) 2019-04, Vol.161, p.50-56
• Main Authors: Kövesi, Benjamin, Pelyhe, Csilla, Zándoki, Erika, Mézes, Miklós, Balogh, Krisztián
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant defence system; Carps - genetics; Carps - metabolism; Common carp; Gene expression; Gene Expression Regulation - drug effects; Genes, Regulator - drug effects; Glutathione - metabolism; Glutathione peroxidase; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Kelch-Like ECH-Associated Protein 1 - metabolism; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - enzymology; Liver - metabolism; Oxidation-Reduction - drug effects; Oxidative Stress - drug effects; RNA - genetics; RNA - metabolism; Sterigmatocystin; Sterigmatocystin - pharmacology
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2019.03.001

Sterigmatocystin (STC) is structurally close to the mycotoxin aflatoxin B1 as it shares its biosynthetic pathway with aflatoxins. The purpose of the present study was to investigate the short-term (24 h) effects of STC contaminated diet at different doses (1 mg, 2 mg and 4 mg STC kg−1 feed) in one year old common carp juveniles. Liver samples were taken in 8-h intervals. The markers of the lipid peroxidation showed moderate changes after the application of sterigmatocystin-contaminated diet, significant elevations were only observed in the lowest applied dose group of sterigmatocystin after 16 h of exposure. Reduced glutathione content showed higher levels than control group after 16 h of exposure as effect of low dose of sterigmatocystin. Glutathione peroxidase (GPX4) activity was lower than control in the group treated with 2 mg STC kg−1 feed after 24 h of exposure. Gene expression measurements of keap1, nrf2, gpx4a, gpx4b and gss genes revealed a dual response. Down-regulation or near control values were observed 8 h after exposure which was followed by an induction 16 and 24 h after exposure. In case of gsr, gene expression values returned to control levels by the 24th hour. In summary, these results suggest that lower doses of STC caused oxidative stress earlier than higher doses, which efficiently activated the Keap1-Nrf2 pathway, while higher doses revealed long-drawn activation of this pathway. •Short term exposure of sterigmatocystin was used in common carp as pro-oxidant effect.•Lipid peroxidation parameters elevated as effect of low dose of STC.•Glutathione redox system slightly responded after STC exposure.•Expression of glutathione redox system regulatory genes showed dual response after STC exposure.•The results indicated that low dose of STC provokes oxidative stress earlier than higher.