Three populations of adult Leydig cells in mouse testes revealed by a novel mouse HSD3B1-specific rat monoclonal antibody

Leydig cells play a pivotal function in the synthesis of a male sex steroid, testosterone. The ability of the steroid production is dependent on the expression of the steroidogenic genes, such as HSD3B (3β-hydroxysteroid dehydrogenase/Δ5- Δ4 isomerase). It has been established that two different typ...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2019-04, Vol.511 (4), p.916-920
Main Authors: Yokoyama, Chikako, Chigi, Yuta, Baba, Takashi, Ohshitanai, Atsushi, Harada, Yumi, Takahashi, Fumiya, Morohashi, Ken-ichirou
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - chemistry
Cell Lineage
Fluorescent Antibody Technique
HSD3B1
HSD3B6
Leydig cells
Leydig Cells - cytology
Male
Mice
Monoclonal antibody
Multienzyme Complexes - analysis
Progesterone Reductase - analysis
Protein Isoforms - analysis
Rats
Steroid Isomerases - analysis
Testis
Testis - cytology
Testis - embryology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leydig cells play a pivotal function in the synthesis of a male sex steroid, testosterone. The ability of the steroid production is dependent on the expression of the steroidogenic genes, such as HSD3B (3β-hydroxysteroid dehydrogenase/Δ5- Δ4 isomerase). It has been established that two different types of Leydig cells, fetal Leydig cells (FLCs) and adult Leydig cells (ALCs), are developed in mammalian testes. FLCs and ALCs are characterized by different sets of marker gene expression. In the case of mouse Leydig cells, Hsd3b1 (Hsd3b type 1) is expressed both in FLCs and ALCs whereas Hsd3b6 (Hsd3b type 6) is expressed in ALCs but not in FLCs. However, because the antibodies established so far for HSD3B were unable to distinguish between the HSD3B1 and HSD3B6 isoforms, it remained unclear whether both of them are expressed in every ALC. Therefore, in the present study, we generated a rat monoclonal antibody specific for mouse HSD3B1. Intriguingly, this monoclonal antibody together with an antibody specific for HSD3B6 identified three populations of ALCs based on the expression levels of these HSD3Bs. •Anti-mouse HSD3B1 rat monoclonal antibody (mAb 3E5) was generated based on the rat lymph node method.•MAb 3E5 bind selectively to mouse HSD3B1 but not HSD3B6.•MAb 3E5 identified three populations of adult Leydig cells based on the expression levels of these HSD3Bs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2019.02.100