Kinetics of Alphatorquevirus plasma DNAemia at late times after allogeneic hematopoietic stem cell transplantation

Torque teno virus (TTV) plasma DNA load has been consistently shown to be a surrogate biomarker of immunosuppression in solid organ transplant recipients. It is uncertain whether it may behave similarly in allogeneic hematopoietic stem cell transplant recipients (allo-HSCT). Here, we characterized t...

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Bibliographic Details
Published in:Medical microbiology and immunology 2019-04, Vol.208 (2), p.253-258
Main Authors: Albert, Eliseo, Solano, Carlos, Giménez, Estela, Focosi, Daniele, Pérez, Ariadna, Macera, Lisa, Piñana, José Luis, Mateo, Eva María, Boluda, Juan Carlos Hernández, Maggi, Fabrizio, Navarro, David
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Corticoids
Corticosteroids
Deoxyribonucleic acid
DNA
Flow cytometry
Graft-versus-host reaction
Hematopoietic stem cells
Immunology
Immunosuppression
Kinetics
Lymphocytes T
Medical Microbiology
Medical treatment
Original Investigation
Patients
Stem cell transplantation
Stem cells
Transplantation
Transplants & implants
Virology
Viruses
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Summary:Torque teno virus (TTV) plasma DNA load has been consistently shown to be a surrogate biomarker of immunosuppression in solid organ transplant recipients. It is uncertain whether it may behave similarly in allogeneic hematopoietic stem cell transplant recipients (allo-HSCT). Here, we characterized the dynamics of TTV DNAemia in patients undergoing T-cell replete allo-SCT at late times after transplantation (> day + 100). This retrospective single-center observational study included 33 allo-HSCT patients. Plasma TTV DNA loads were quantified by real-time PCR before initiating the conditioning regimen and at different time points after transplant. Absolute lymphocyte counts (ALC) were measured by flow cytometry. Overall, TTV DNA load increased steadily after engraftment, reaching a peak by day + 90; afterwards, it remained relatively constant until day + 210. TTV DNA loads measured within days + 120 and + 210 correlated inversely with paired ALC, while both parameters did correlate directly within days + 20 and + 60. The median TTV DNA area under a curve between days + 90 and + 210 [(AUC) 90–210 ] was significantly higher in patients who received corticosteroids within this time frame for treatment of graft versus host disease (either acute, chronic or both) than in controls ( P  = 0.025). In summary, TTV DNA load may mirror the degree of immunosuppression at late times after allo-HSCT.
ISSN:0300-8584
1432-1831
DOI:10.1007/s00430-019-00586-w