Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii

[Display omitted] •A new cypovirus from Daphnis nerii cypovirus type 23 (DnCPV-23) is identified.•All genomic segments of DnCPV, except segment 4, have conserved termini.•Electrophoretic pattern and the termini are different from 22 other CPV types.•DnCPV is most similar to CPV-5 and CPV-19. The ole...

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Bibliographic Details
Published in:Journal of invertebrate pathology 2019-05, Vol.163, p.43-47
Main Authors: Zhan, Zhigao, Guan, Limei, Wang, Jinchang, Liu, Zhuorong, Guo, Yan, Xiao, Yuzhou, Wang, Hongxiu, Jin, Liang
Format: Article
Language:English
Subjects:
Animals
Cypovirus
Daphnis nerii
Full-length amplification of cDNA
Genome, Viral
Genomic characterization
Insect Viruses - classification
Insect Viruses - genetics
Insect Viruses - isolation & purification
Insect Viruses - ultrastructure
Moths - virology
Phylogeny
Reoviridae - classification
Reoviridae - genetics
Reoviridae - isolation & purification
Reoviridae - ultrastructure
Viral Proteins - genetics
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Summary:[Display omitted] •A new cypovirus from Daphnis nerii cypovirus type 23 (DnCPV-23) is identified.•All genomic segments of DnCPV, except segment 4, have conserved termini.•Electrophoretic pattern and the termini are different from 22 other CPV types.•DnCPV is most similar to CPV-5 and CPV-19. The oleander hawk moth, Daphnis nerii, is a serious pest of plants belonging to the family Apocynaceae. Thus far, pathogen infection has not been reported in D. nerii. In this study, a new cytoplasmic polyhedrosis virus (cypovirus; CPV) was isolated from naturally diseased D. nerii larvae and named DnCPV-23. Virions were observed in ultrathin sections of DnCPV polyhedral bodies. Electrophoretic analysis revealed that the DnCPV genome consisted of 10 segments of double-stranded RNA (dsRNA). cDNA copies of these dsRNA segments were amplified using the method of full-length amplification of cDNAs (FLAC), cloned, and sequenced. Sequencing results showed that all segments contained one open reading frame (ORF); They shared the conserved terminal sequences AGUCAAA and AGC at 5′ and 3′ ends respectively, except segment 4, which is different from previously reported 22 cypoviruses. Phylogenetic analysis based on amino acid sequences of polyhedrin (encoded by segment 10) indicated that this CPV was closely related to CPV type 19. Altogether, DnCPV-23 is a new type of cypovirus.
ISSN:0022-2011
1096-0805
DOI:10.1016/j.jip.2019.03.002