The inhibition of accessory gene regulator C specific binding peptides on biofilm formation of Staphylococcus epidermidis on the surface of polyvinyl chloride in vitro

• Published in: Zhongguo xiu fu chong jian wai ke za zhi 2019-03, Vol.33 (3), p.349
• Main Authors: Yang, Jichen, Qiu, Liangting, Huang, Yunchao, Chen, Ya, Rao, Sunyin, Ruan, Wenpeng, Zhao, Guangqiang, Ye, Lianhua
• Format: Article
• Language: Chinese
• Subjects: Biofilms; Genes, Bacterial; Genes, Regulator; Microscopy, Electron, Scanning; Peptides - pharmacology; Polyvinyl Chloride; Staphylococcus epidermidis - growth & development
• ISSN: 1002-1892
• DOI: 10.7507/1002-1892.201806110

To investigate the effect of accessory gene regulator C (agr C) specific binding peptides (named N1) on the biofilm formation of on the surface of polyvinyl chloride (PVC) materials . Firstly, the two strains (ATCC35984, ATCC12228) were cultured with N1 at concentrations of 100, 200, 400, 800, and 1 600 μg/mL, respectively. The control group was cultured with agrC specific binding unrelated peptides (named N0) at the same concentrations and the absorbance ( ) value was measured after 24 hours to determine the optimal bacteriostatic concentration of N1. The two strains were cultured with N1 and N0 of the optimal concentration, respectively. The values were measured at 6, 12, 18, 24, 30, and 48 hours to observe the effect of N1 on the biofilm formation ability of . On this basis, the surface structure of the biofilm on the surface of PVC material was observed by scanning electron microscopy after 6, 12, 18, 24, and 30 hours of incubation with PVC material sheet. The thickness of the biofilm was observed by lase