The hematopoietic organ of Macrobrachium rosenbergii: Structure, organization and immune status

• Published in: Fish & shellfish immunology 2019-05, Vol.88, p.415-423
• Main Authors: Pudgerd, Arnon, Chotwiwatthanakun, Charoonroj, Kruangkum, Thanapong, Itsathitphaisarn, Ornchuma, Sritunyalucksana, Kallaya, Vanichviriyakit, Rapeepun
• Format: Article
• Language: English
• Subjects: Animals; Arthropod Proteins - chemistry; Hematopoietic Stem Cells; Hematopoietic System - cytology; Hematopoietic System - immunology; Hematopoietic tissue; Hemocytes - immunology; Hemolymph; Hemolymph vessel; Macrobrachium rosenbergii; Palaemonidae - anatomy & histology; Palaemonidae - immunology; Phagocytosis; Proliferating Cell Nuclear Antigen - chemistry; Stem cell
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2019.03.011

The hematopoietic organ (HO) of the giant freshwater prawn Macrobrachium rosenbergii is a discrete, whitish mass located in the epigastric region of the cephalothorax, posterior to the brain. It is composed of hematopoietic cells arranged in a thick layer of numerous lobules that surround a central hemal sinus from which they are separated by a thin sheath. At the center of the sinus is the muscular cor frontale. The lobules extend radially outward from the sinus in three developmental zones. Basal Zone 1 nearest the sinus contains large hematopoietic stem cells with euchromatic nuclei that stain positive for proliferation cell nuclear antigen (PCNA). Zone 2 contains smaller, actively dividing cells as indicated by positive 5-bromo-20-deoxyuridine (BrdU) staining. Distal Zone 3 contains small, loosely packed cells with heterochromatic nuclei, many cytoplasmic granules and vesicles indicating that they will eventually differentiate into hemocytes and enter circulation. Three main arteries, namely the ophthalmic and the 2 branches of the antennary, connect the heart to the HO. Use of India ink and 0.1 μm fluorescent micro-beads injected into the heart revealed that the cor frontale could immediately remove foreign particles from hemolymph by filtration. Fluorescent beads were also detected in the hematopoietic tissue at 30 min after injection, indicating that it could be penetrated by foreign particles. However, the fluorescent signal completely disappeared from the whole HO after 4 h, indicating its role in removal of foreign particles. In conclusion, the present study demonstrated for the first time the detailed histological structures of the HO of M. rosenbergii and its relationship to hematopoiesis and removal of foreign particles from hemolymph. •First detailed description of the hematopoietic organ of M. rosenbergii.•The hematopoietic organ is under the rostrum base, posterior to the brain and closely associated with the cor frontale.•The cor frontale sinus is an effective trap for foreign particles in hemolymph.•The HO can recognize and eliminate foreign particles.