Neurocysticercosis serodiagnosis: mimotope-based synthetic peptide as potential biomarker

Herein, we evaluate a mimotope-based synthetic peptidenamed NC 4 1 to diagnose neurocysticercosis (NC), a neglected parasitic disease and a major cause of epilepsy worldwide. NC 4 1 synthetic peptide was evaluated to diagnose NC, and total saline extract from Taenia solium metacestodes (SE) was used...

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Bibliographic Details
Published in:Parasitology research (1987) 2019-05, Vol.118 (5), p.1657-1660
Main Authors: Ribeiro, Vanessa da Silva, Gonzaga, Henrique Tomaz, Nunes, Daniela da Silva, Goulart, Luiz Ricardo, Costa-Cruz, Julia Maria
Format: Article
Language:English
Subjects:
Antigenic determinants
Biomedical and Life Sciences
Biomedicine
Cysticercosis
Diseases
Epilepsy
Epitopes
Health risk assessment
Immunology
Immunology and Host-Parasite Interactions - Short Communication
Invertebrates
Medical Microbiology
Microbiology
Parasitic diseases
Peptides
Phosphates
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Summary:Herein, we evaluate a mimotope-based synthetic peptidenamed NC 4 1 to diagnose neurocysticercosis (NC), a neglected parasitic disease and a major cause of epilepsy worldwide. NC 4 1 synthetic peptide was evaluated to diagnose NC, and total saline extract from Taenia solium metacestodes (SE) was used as control. Serum samples from patients with NC ( n  = 40), other parasitic diseases ( n  = 43), and healthy individuals ( n  = 40) were tested. Diagnostic parameters such as sensitivity (Se), specificity (Sp), likelihood ratio (LR), and area under curve (AUC) were calculated using receiver operating characteristic (ROC) curves. The sequence from T. solium phosphoenolpyruvate carboxykinase (PEPCK) was used for epitope prediction, resulting in one high-scoring patch centered at residue L247. NC 4 1 synthetic peptide reached high diagnostic performance (Se 97.5% and Sp 97.5%, LR+ 39 and AUC 0.997). Data from diagnostic parameters and in silico analyses proved the usefulness of NC 4 1 synthetic peptide as a diagnostic marker for human NC.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-019-06288-9