A novel human monoclonal Trop2-IgG antibody inhibits ovarian cancer growth in vitro and in vivo

• Published in: Biochemical and biophysical research communications 2019-04, Vol.512 (2), p.276-282
• Main Authors: Liu, Jinrong, Yang, Dazhen, Yin, Zhengna, Gao, Mengyun, Tong, Hua, Su, Yiping, Zhu, Jin, Ye, Chunping, Zhang, Huilin
• Format: Article
• Language: English
• Subjects: Animals; Antibody; Antigens, Neoplasm - genetics; Antigens, Neoplasm - immunology; Antineoplastic Agents, Immunological - therapeutic use; Apoptosis - drug effects; Cell Adhesion Molecules - antagonists & inhibitors; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - immunology; Cell Line, Tumor; Female; Humans; IgG; Immunoglobulin G - genetics; Immunoglobulin G - therapeutic use; Immunotherapy; Mice; Mice, Nude; Neoplasm Invasiveness - pathology; Neoplasm Invasiveness - prevention & control; Ovarian Neoplasms - immunology; Ovarian Neoplasms - pathology; Ovarian Neoplasms - therapy; Trop2; Xenograft Model Antitumor Assays
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2019.03.028

Trop2 is a tumor-related antigen closely related to the development of a variety of tumors and has been identified as a promising target for cancer immunotherapy. In this study, a Trop2-IgG antibody was constructed by a eukaryotic expression system based on our previously constructed Trop2-Fab antibody. SDS-PAGE, cell ELISA, affinity assays, fluorescence staining and FACS analyses were performed to characterize Trop2-IgG. Then, CCK-8, wound healing, Transwell and annexin V-PI assays were employed to evaluate the tumor inhibitory effects of Trop2-IgG on OC in vitro, while tumor-bearing mice were constructed to examine the tumor inhibitory effects of Trop2-IgG on OC in vivo. Trop2-IgG was successfully constructed by a eukaryotic expression system and maintained recognition characteristics to Trop2 antigen. In vitro, Trop2-IgG could inhibited tumor cell growth, migration, and invasion compared to those of control cells and induced tumor cell apoptosis. In vivo, Trop2-IgG exerted critical tumor inhibitory effects in OC xenografts. Our data suggest that the use of Trop2-IgG provides a potential therapeutic strategy for the immunotherapy of Trop2-expressing OC. •A Trop2-IgG antibody was constructed by a eukaryotic expression system based on previous Trop2-Fab antibody.•Trop2-IgG inhibited tumor cell growth, migration, invasion and induced tumor cell apoptosis in OC cell lines.•Trop2-IgG exerted tumor inhibitory effects in OC xenografts.•Trop2-IgG is a strong therapeutic strategy for the immunotherapy of Trop2-expressing OC.