Trimetallic signal amplification aptasensor for TSP-1 detection based on Ce-MOF@Au and AuPtRu nanocomposites

• Published in: Biosensors & bioelectronics 2019-05, Vol.132, p.302-309
• Main Authors: Fu, Xiaoxue, He, Junlin, Zhang, Chengli, Chen, Jun, Wen, Yilin, li, Jia, Mao, Weiran, Zhong, Hangtian, Wu, Jiahao, Ji, Xingduo, Yu, Chao
• Format: Article
• Language: English
• Subjects: Aptamers, Nucleotide - chemistry; Biosensing Techniques - methods; Ce-based metal-organic framework; Cerium - chemistry; Electrochemical aptasensor; Electrochemical Techniques - methods; Gold - chemistry; Humans; Immobilized Nucleic Acids - chemistry; Limit of Detection; Metal-Organic Frameworks - chemistry; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Platinum - chemistry; Ruthenium - chemistry; Thrombospondin 1 - analysis; Thrombospondin 1 - blood; Thrombospondin-1; Trimetallic nanocomposite
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2019.02.054

In this work, an aptamer was used as the target capturing agent and a trimetallic signal amplification strategy based on Ce-MOF@Au and AuPtRu NPs was demonstrated for the sensitive detection of TSP-1. Herein, the synthesized AuPtRu nanocomposite (AuPtRu NPs) not only acts as the catalyst for catalyzing hydrogen peroxide but also acts as a nanocarrier for capturing the -NH2 termination single strand DNA (S1) to obtain the signal probe (SP, AuPtRu nanocomposite/S1). Then, SP was efficiently linked into TSP-1 aptamers with the addition of complementary linking strands to form M1 (SP/aptamer). The Ce-MOF@Au nanocomposites were obtained by in situ reduction and used as GCE electrode modification materials. The -NH2-modified capture probe (CP) DNA was immobilized on the surface of Ce-MOF@Au nanocomposites for hybridizing SP. In the presence of the target TSP-1, the aptamer recognizes the target and binds strongly so that SP is released from the prepared M1 and then hybridized with CP. When the detection solution contains an electrochemical matrix of H2O2, AuPtRu NPs can oxidize H2O2 to obtain an enhanced signal. Furthermore, the proposed aptasensor has a very low LOD of 0.13 fg mL−1 TSP-1 in the detection range of 1 fg mL−1 to 10 ng mL−1. Moreover, the proposed platform also has application implications for other potential targets. •Aptasensor for TSP-1 detection in the human serum samples can be achieved.•Aptamer and complement recognition as a bispecific recognition mechanism.•Trimetallic AuPtRu NPs was synthesized and showed excellent catalytic ability.•Synthesizing Ce-MOF@Au by one-pot method used as electrode modification materials.