Genetic diversity in Corchorus olitorius L. revealed by morphophysiological and molecular analyses

• Published in: Molecular biology reports 2019-06, Vol.46 (3), p.2933-2940
• Main Authors: Youssef, Ahmed Fathy, Younes, Nabil Ahmed, Youssef, Muhammad
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Antioxidants; Biomedical and Life Sciences; Corchorus - genetics; Corchorus olitorius; Egypt; Gene polymorphism; Genetic analysis; Genetic diversity; Genetic relationship; Genetic Variation - genetics; Genotype; Histology; Life Sciences; Morphology; Nucleotide sequence; Original Article; Phenotype; Phylogeny; Plant breeding; Plant Breeding - methods; Plant Leaves - genetics; Polymerase chain reaction; Random amplified polymorphic DNA; Random Amplified Polymorphic DNA Technique - methods; Vitamins
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-019-04754-2

Assessment of genetic diversity has an efficient role in plant breeding and improvement programs. There is a limit number of investigations dealing with the evaluation of genetic diversity in Jew’s mallow ( Corchorus olitorius L.), despite its valuable importance as a leafy vegetable and a delicious dish rich in vitamins and antioxidants. Therefore, in this study, 18 landraces of Jew’s mallow—collected from different locations in Egypt—were used for genetic diversity assessment based on morphophysiological and molecular evaluations. A high degree of variability was found among the evaluated landraces at both levels, indicating the appropriateness of such collection to be involved in breeding approaches. Some morphophysiological traits offered a high level of diversity and effectively discriminated the landraces. Thus, they are recommended to be used in successive morphological evaluation studies. On the other hand, molecular evaluation using the random amplified polymorphic DNA (RAPD) and the sequence related amplified polymorphism (SRAP) efficiently supported the morphological results by exposing a clear genetic relationship among the landraces. In addition, the principal coordinate analysis based on combined data of RAPD and SRAP divided the landraces into two main groups, reflecting their relationship molecularly. The first group included nine landraces related to Upper Egypt and the second gathered three landraces from Delta, while the other six landraces were distinctly distributed around these two groups. The two groups may have two distinct ancestors in addition to the different ancestors of the scattered landraces. Findings of this study are valuable and could assist in Jew’s mallow breeding programs.