Microflora of fresh white button mushrooms (Agaricus bisporus) during cold storage revealed by high‐throughput sequencing and MALDI‐TOF mass spectrometry fingerprinting

• Published in: Journal of the science of food and agriculture 2019-07, Vol.99 (9), p.4498-4503
• Main Authors: Qiu, Wanwei, Huang, Ying, Zhao, Chao, Lin, Zhenshan, Lin, Wenxing, Wang, Zejin
• Format: Article
• Language: English
• Subjects: Agaricus - chemistry; Agaricus bisporus; Bacteria - chemistry; Bacteria - genetics; Bacteria - growth & development; Bacteria - isolation & purification; Basidiocarps; Bioassays; Brown blotch; Cold storage; Communities; Fingerprinting; Food Storage; Gene sequencing; High-Throughput Nucleotide Sequencing - methods; high‐throughput sequencing; MALDI‐TOF MS; Mass spectrometry; Mass spectroscopy; Microbial activity; microbial communities; Microbiota; Microflora; Microorganisms; Mushrooms; Organic chemistry; Pathogenicity; Pathogens; Pseudomonas; Relative abundance; rRNA 16S; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectroscopy; Tandem Mass Spectrometry - methods; Vegetables - chemistry; Vegetables - microbiology
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.9695

ABSTRACT BACKGROUND Fresh Agaricus bisporus is popular and consumed throughout the world because of its taste, as well as its nutritional and medicinal properties, but it is also prone to microbial growth. There is very limited information about the dynamic changes of microbial communities during storage. The present study aimed to analyze the microbial diversity of button mushroom during cold storage using Illumina HiSeq sequencing. Bacteria isolated from the later storage period were identified by MALDI‐TOF mass spectrometry and a bioassay of pathogenicity was carried out. RESULTS High‐throughput sequencing showed that Pseudomonas was the predominant genus throughout the storage period. Pedobacter and Flavobacterium grew prolifically on the eighth day, while the relative abundance of Oscillospira continued to decrease. Pseudomonas, Ewingella and Chryseobacterium were isolated at the later period of mushroom storage. A pathogenicity bioassay on the cap of mushrooms showing brown blotch indicated an infection by Pseudomonas tolaasii. However, Ewingella americana did not have a pathogenic effect in our study. CONCLUSION Bacterial communities of fresh Agaricus bisporus during cold storage were characterized by high‐throughput sequencing. MALDI‐TOF MS provides a good analytical procedure, in addition to 16S rRNA gene sequencing. © 2019 Society of Chemical Industry