Mapping of barley susceptibility/resistance QTL against spot form net blotch caused by Pyrenophora teres f. maculata using RIL populations

• Published in: Theoretical and applied genetics 2019-07, Vol.132 (7), p.1953-1963
• Main Authors: Tamang, Prabin, Richards, Jonathan K., Alhashal, Abdullah, Sharma Poudel, Roshan, Horsley, Richard D., Friesen, Timothy L., Brueggeman, Robert S.
• Format: Article
• Language: English
• Subjects: Agriculture; Analysis; Barley; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cultivars; Disease susceptibility; Gene mapping; Gene polymorphism; Genetic polymorphisms; Inbreeding; Life Sciences; Net blotch; Original Article; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Polymerase chain reaction; Pyrenophora teres; Quantitative genetics; Quantitative trait loci; Seedlings; Single-nucleotide polymorphism; Spot blotch; Traditions
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-019-03328-x

Spot form net blotch (SFNB) caused by the necrotrophic fungal pathogen Pyrenophora teres f. maculata ( Ptm ) is an important disease of barley worldwide including the major barley production regions of North America. To characterize SFNB resistance/susceptibility quantitative trait loci (QTL), three recombinant inbred line (RIL) populations were developed from crosses between the malting barley cultivars, Tradition (six row) and Pinnacle (two row), and the two world barley core collection lines, PI67381 and PI84314. Tradition and Pinnacle were susceptible to many North American Ptm isolates, while PI67381 and PI84314 carry resistances to diverse Ptm isolates from across the globe. The RIL populations, Tradition/PI67381, Pinnacle/PI67381, and Pinnacle/PI84314 were genotyped using polymerase chain reaction-mediated genotype-by-sequencing single nucleotide polymorphism marker panels and phenotyped at the seedling stage with six geographically distinct Ptm isolates: FGOB10Ptm-1 (North Dakota, USA), Pin-A14 (Montana, USA), Cel-A17 (Montana, USA), SG1 (Australia), NZKF2 (New Zealand) and DEN2.6 (Denmark). The goal was to determine if the susceptible elite lines contained common susceptibility genes/QTL or if the resistant lines had common resistant genes/QTL effective against diverse Ptm isolates. The QTL analyses identified a total of 12 resistance and/or susceptibility loci on chromosomes 2H, 3H, 4H, 6H, and 7H of which three had not been previously reported. Common major QTL were detected on chromosome 2H ( R 2  = 14–40%) and 7H ( R 2  = 24–80%) in all three RIL populations, suggesting underlying genes with broad resistance specificity. The major 7H QTL was shown to be a dominant susceptibility gene in both susceptible malting barley varieties.