LncRNA NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation

The aim of this study was to explore the role of NRON in the atrial fibrosis. The expression of NRON in atrial tissue was detected using qRT-PCR. The protein levels of collagen I, collagen III, NFATc3 and p-NFATc3 were determined by western blot. Immunohistochemistry assay were performed to observe...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2019-07, Vol.457 (1-2), p.169-177
Main Authors: Wang, Yapeng, Xu, Peng, Zhang, Chengxin, Feng, Junbo, Gong, Wenhui, Ge, Shenglin, Guo, Zhixiang
Format: Article
Language:English
Subjects:
Adult
Animals
Biochemistry
Biomedical and Life Sciences
Calcium-binding protein
Cardiology
Cholecystokinin
Collagen
Collagen (type I)
Collagen (type III)
Collagen Type I - biosynthesis
Collagen Type III - biosynthesis
Female
Fibroblasts
Fibroblasts - metabolism
Fibroblasts - pathology
Fibrosis
Heart Atria - metabolism
Heart Atria - pathology
Humans
Immunofluorescence
Immunohistochemistry
Kinases
Life Sciences
Male
Medical Biochemistry
Mice
Middle Aged
Myocardium - metabolism
Myocardium - pathology
NFATC Transcription Factors - metabolism
Oncology
Phosphorylation
Proteins
RNA, Long Noncoding - metabolism
Staining
Troponin
Vimentin
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Summary:The aim of this study was to explore the role of NRON in the atrial fibrosis. The expression of NRON in atrial tissue was detected using qRT-PCR. The protein levels of collagen I, collagen III, NFATc3 and p-NFATc3 were determined by western blot. Immunohistochemistry assay were performed to observe expression and distribution of collagen I in atrial tissues. The atrial fibroblasts were authenticated by vimentin/troponin immunofluorescence staining. Fibroblast proliferation was detected by CCK-8 assay. The morphological changes of cardiac tissue were observed by HE staining. Myocardial fibrosis was detected by masson staining. NRON expression was significantly downregulated in atrial tissues of AF. NRON suppressed fibroblast proliferation; expression of collagen I and collagen III; activation of NFATc3 and nucleu import. NRON promoted p-NFATc3 expression and alleviated atrial fibrosis in vivo. Our data indicated that NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-019-03521-y