Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells from the Apical Side of the Membrane under Static and Microcirculation Conditions

Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulating natural environment of the body in comparison with static culturing. Impedance spectrosco...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2019-03, Vol.166 (5), p.626-630
Main Authors: Nikulin, S. V., Gerasimenko, T. N., Shilin, S. A., Gazizov, I. N., Kindeeva, O. V., Sakharov, D. A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cell Biology
Homunculus
Inflammation
Internal Medicine
Ions
Laboratory Medicine
Laminin
Microfluidics
MicroRNAs
miRNA
Pathology
Secretion
Spectroscopy
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Summary:Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulating natural environment of the body in comparison with static culturing. Impedance spectroscopy (BioClinicum Research Center) was used for non-invasive estimation of the monolayer quality and changes in the cell apical membrane due to the formation of microvilli. Under static conditions, Caco-2 cells release more microRNA from the apical membrane than under microcirculation conditions, while secretion of miR-320a, miR-24-3p, and miR-221-3p microRNA under static conditions can indicate stress of the cells and activation of inflammatory response. Under microcirculation conditions, the expression of laminin-α1 (LAMA1) was lower than under static conditions, which indicates deeper differentiation of cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-019-04406-2