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Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds
BACKGROUND Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compou...
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| Published in: | Journal of the science of food and agriculture 2019-08, Vol.99 (10), p.4677-4685 |
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| cites | cdi_FETCH-LOGICAL-c3538-96e9abee21fcc33f5c9822f28d58064629c310c5647454ab8d72aa83377a03f23 |
| container_end_page | 4685 |
| container_issue | 10 |
| container_start_page | 4677 |
| container_title | Journal of the science of food and agriculture |
| container_volume | 99 |
| creator | Glibota, Nicolás Grande Burgos, Mª José Gálvez, Antonio Ortega, Elena |
| description | BACKGROUND
Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics.
RESULTS
Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole.
CONCLUSION
Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.9708 |
| format | article |
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Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics.
RESULTS
Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole.
CONCLUSION
Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.9708</identifier><identifier>PMID: 30906996</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Agricultural land ; Ampicillin ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Antimicrobial resistance ; Bacteria ; Bacteria - classification ; Bacteria - drug effects ; Bacteria - genetics ; Bacteria - isolation & purification ; Burkholderia ; Copper ; Copper - pharmacology ; Copper compounds ; copper tolerance ; Drug resistance ; Drug Resistance, Bacterial ; Erythromycin ; Fruit trees ; Heavy metals ; Lead - pharmacology ; metal tolerance ; Microbial Sensitivity Tests ; Nickel ; Nickel - pharmacology ; Olea - growth & development ; olive tree fields ; Organic chemistry ; Pseudomonas ; Soil - chemistry ; Soil bacteria ; Soil Microbiology ; Soil microorganisms ; Soil resistance ; Soils ; Strains (organisms) ; Sulfamethoxazole ; Trees ; Trimethoprim ; Vancomycin ; Zinc ; Zinc - pharmacology</subject><ispartof>Journal of the science of food and agriculture, 2019-08, Vol.99 (10), p.4677-4685</ispartof><rights>2019 Society of Chemical Industry</rights><rights>2019 Society of Chemical Industry.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-96e9abee21fcc33f5c9822f28d58064629c310c5647454ab8d72aa83377a03f23</citedby><cites>FETCH-LOGICAL-c3538-96e9abee21fcc33f5c9822f28d58064629c310c5647454ab8d72aa83377a03f23</cites><orcidid>0000-0002-5894-5029</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30906996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Glibota, Nicolás</creatorcontrib><creatorcontrib>Grande Burgos, Mª José</creatorcontrib><creatorcontrib>Gálvez, Antonio</creatorcontrib><creatorcontrib>Ortega, Elena</creatorcontrib><title>Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics.
RESULTS
Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole.
CONCLUSION
Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry</description><subject>Agricultural land</subject><subject>Ampicillin</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Burkholderia</subject><subject>Copper</subject><subject>Copper - pharmacology</subject><subject>Copper compounds</subject><subject>copper tolerance</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>Erythromycin</subject><subject>Fruit trees</subject><subject>Heavy metals</subject><subject>Lead - pharmacology</subject><subject>metal tolerance</subject><subject>Microbial Sensitivity Tests</subject><subject>Nickel</subject><subject>Nickel - pharmacology</subject><subject>Olea - growth & development</subject><subject>olive tree fields</subject><subject>Organic chemistry</subject><subject>Pseudomonas</subject><subject>Soil - chemistry</subject><subject>Soil bacteria</subject><subject>Soil Microbiology</subject><subject>Soil microorganisms</subject><subject>Soil resistance</subject><subject>Soils</subject><subject>Strains (organisms)</subject><subject>Sulfamethoxazole</subject><subject>Trees</subject><subject>Trimethoprim</subject><subject>Vancomycin</subject><subject>Zinc</subject><subject>Zinc - pharmacology</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU1vFDEMhiNERZfCgT-AInGBw7ROMl85VivKhyr1UDhHmYwDWWUmQ5Jptb-Av022Wzgg9WDZkh-_tvwS8obBOQPgF7tk9bnsoH9GNgxkVwEweE42pcerhtX8lLxMaQcAUrbtC3IqQEJb6g35vQ3LgpHm4DHq2SDV81giu8GF7AyNmFzKDx030xScp4M2GaPT1MYw0eDdHdIcsYz-iM6sPq9Re2od-jHRGNbsZvT7A6IzjvTe5Z_UHNeaMC1hncf0ipxY7RO-fsxn5PvVx2_bz9X1zacv28vryohG9JVsUeoBkTNrjBC2MbLn3PJ-bHpo65ZLIxiYpq27uqn10I8d17oXous0CMvFGXl_1F1i-LViympyyaD3esawJsWZ7IQAwaGg7_5Dd2GNc7lOcV7z8swe-kJ9OFImhpQiWrVEN-m4VwzUwR11cEcd3Cns20fFdZhw_Ef-taMAF0fg3nncP62kvt5eXT5I_gG3d5uK</recordid><startdate>20190815</startdate><enddate>20190815</enddate><creator>Glibota, Nicolás</creator><creator>Grande Burgos, Mª José</creator><creator>Gálvez, Antonio</creator><creator>Ortega, Elena</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5894-5029</orcidid></search><sort><creationdate>20190815</creationdate><title>Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds</title><author>Glibota, Nicolás ; Grande Burgos, Mª José ; Gálvez, Antonio ; Ortega, Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-96e9abee21fcc33f5c9822f28d58064629c310c5647454ab8d72aa83377a03f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agricultural land</topic><topic>Ampicillin</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Burkholderia</topic><topic>Copper</topic><topic>Copper - pharmacology</topic><topic>Copper compounds</topic><topic>copper tolerance</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial</topic><topic>Erythromycin</topic><topic>Fruit trees</topic><topic>Heavy metals</topic><topic>Lead - pharmacology</topic><topic>metal tolerance</topic><topic>Microbial Sensitivity Tests</topic><topic>Nickel</topic><topic>Nickel - pharmacology</topic><topic>Olea - growth & development</topic><topic>olive tree fields</topic><topic>Organic chemistry</topic><topic>Pseudomonas</topic><topic>Soil - chemistry</topic><topic>Soil bacteria</topic><topic>Soil Microbiology</topic><topic>Soil microorganisms</topic><topic>Soil resistance</topic><topic>Soils</topic><topic>Strains (organisms)</topic><topic>Sulfamethoxazole</topic><topic>Trees</topic><topic>Trimethoprim</topic><topic>Vancomycin</topic><topic>Zinc</topic><topic>Zinc - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Glibota, Nicolás</creatorcontrib><creatorcontrib>Grande Burgos, Mª José</creatorcontrib><creatorcontrib>Gálvez, Antonio</creatorcontrib><creatorcontrib>Ortega, Elena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Glibota, Nicolás</au><au>Grande Burgos, Mª José</au><au>Gálvez, Antonio</au><au>Ortega, Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2019-08-15</date><risdate>2019</risdate><volume>99</volume><issue>10</issue><spage>4677</spage><epage>4685</epage><pages>4677-4685</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics.
RESULTS
Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole.
CONCLUSION
Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>30906996</pmid><doi>10.1002/jsfa.9708</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5894-5029</orcidid></addata></record> |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Agricultural land Ampicillin Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antimicrobial resistance Bacteria Bacteria - classification Bacteria - drug effects Bacteria - genetics Bacteria - isolation & purification Burkholderia Copper Copper - pharmacology Copper compounds copper tolerance Drug resistance Drug Resistance, Bacterial Erythromycin Fruit trees Heavy metals Lead - pharmacology metal tolerance Microbial Sensitivity Tests Nickel Nickel - pharmacology Olea - growth & development olive tree fields Organic chemistry Pseudomonas Soil - chemistry Soil bacteria Soil Microbiology Soil microorganisms Soil resistance Soils Strains (organisms) Sulfamethoxazole Trees Trimethoprim Vancomycin Zinc Zinc - pharmacology |
| title | Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds |
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