Extraction of valuable compounds from Arthrospira platensis using pulsed electric field treatment

• Published in: Bioresource technology 2019-07, Vol.283, p.207-212
• Main Authors: Jaeschke, Débora Pez, Mercali, Giovana Domeneghini, Marczak, Ligia Damasceno Ferreira, Müller, Georg, Frey, Wolfgang, Gusbeth, Christian
• Format: Article
• Language: English
• Subjects: Antioxidant capacity; Cyanobacteria; Electroporation; Phycocyanin; Pigments; Protein; Pulsed electric field
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.03.035

•PEF-treatment induced a damage of the cell structure.•After PEF-treatment, an incubation time was needed for the extraction.•A purified phycocyanin-protein rich extract was obtained.•The antioxidant activity of PEF-extracts was higher than bead milling extracts. Pulsed electric field (PEF) treatment was evaluated for phycocyanin and proteins extraction from Arthrospira platensis (Spirulina platensis). PEF extractions were performed using different specific energies (28, 56 and 122 J·ml−1 of suspension) and the results were compared to the extraction with bead milling. At highest PEF-treatment energies a damage of the cell morphology could be observed and the highest yields (up to 85.2 ± 5.7 mg·g−1 and 48.4 ± 4.4 g·100 g−1 of phycocyanins and proteins, respectively) could be obtained at 122 and 56 J·ml−1. The yields increased with incubation time after PEF-treatment. The antioxidant capacity of the extracts obtained after PEF-treatment was higher than of those obtained after bead milling. PEF treatment is a promising technology to obtain blue-green antioxidant extracts from A. platensis in an environmental friendly process.