PET imaging of 11C-labeled coenzyme Q10: Comparison of biodistribution between [11C]ubiquinol-10 and [11C]ubiquinone-10

Coenzyme Q10 (CoQ10) plays a key role not only as an essential electron carrier in the mitochondrial electron transport chain, but also as an antioxidant to protect cells from oxidative stress. CoQ10 supplementation is expected to be effective for a variety of diseases. The predominant forms of CoQ1...

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Published in:Biochemical and biophysical research communications 2019-05, Vol.512 (3), p.611-615
Main Authors: Watanabe, Kyosuke, Nozaki, Satoshi, Goto, Miki, Kaneko, Ken-ichi, Hayashinaka, Emi, Irie, Satsuki, Nishiyama, Akira, Kasai, Kazuaki, Fujii, Kenji, Wada, Yasuhiro, Mizuno, Kei, Mizuseki, Kenji, Doi, Hisashi, Watanabe, Yasuyoshi
Format: Article
Language:English
Subjects:
Biodistribution
Coenzyme Q10
Metabolism
PET
Ubiquinol
Ubiquinone
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Summary:Coenzyme Q10 (CoQ10) plays a key role not only as an essential electron carrier in the mitochondrial electron transport chain, but also as an antioxidant to protect cells from oxidative stress. CoQ10 supplementation is expected to be effective for a variety of diseases. The predominant forms of CoQ10 are the ubiquinol-10 (reduced form) and ubiquinone-10 (oxidized form). Both forms of CoQ10 supplements are commercially available, however, their kinetic difference is still unclear. In order to conduct in vivo analysis of the kinetics of ubiquinol-10 and ubiquinone-10, we succeeded in synthesizing 11C-labeled ubiquinol-10 ([11C]UQL) and ubiquinone-10 ([11C]UQN), respectively. In the present study, we aimed to investigate the kinetics of [11C]UQL and [11C]UQN, both of which were administered via the tail vein of 8-week-old male Sprague-Dawley rats. Whole-body positron emission tomography (PET) imaging was performed to follow the time course of accumulation in the liver, spleen, brain, and other organs. Then, at the two typical time points at 20 or 90 min after injection, we conducted the biodistribution study. Various organs/tissues and blood were collected, weighed and counted with a gamma counter. Percent injected dose per gram of tissue (%ID/g) was calculated as the indicator of the accumulation of each compound. As the results, at both time points, %ID/g of [11C]UQL in the cerebrum, cerebellum, white adipose tissue, muscle, kidney, and testis were higher (P 
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2019.03.073