Beneficial effects of Spirulina platensis, voluntary exercise and environmental enrichment against adolescent stress induced deficits in cognitive functions, hippocampal BDNF and morphological remolding in adult female rats

• Published in: Hormones and behavior 2019-06, Vol.112, p.20-31
• Main Authors: Moradi-Kor, Nasroallah, Ghanbari, Ali, Rashidipour, Hadi, Yousefi, Behpour, Bandegi, Ahmad Reza, Rashidy-Pour, Ali
• Format: Article
• Language: English
• Subjects: Adolescent stress; Animals; Animals, Newborn; Avoidance Learning - drug effects; Avoidance Learning - physiology; Brain-Derived Neurotrophic Factor - metabolism; Cell Extracts - pharmacology; Cognition - drug effects; Cognition - physiology; Cognition Disorders - etiology; Cognition Disorders - pathology; Cognition Disorders - physiopathology; Cognition Disorders - prevention & control; Cognition exercise; Conditioning, Psychological - drug effects; Conditioning, Psychological - physiology; Dendritic remodeling; Environmental enrichment; Female; Hippocampus - drug effects; Hippocampus - metabolism; Hippocampus - pathology; Hippocampus - physiopathology; Memory - drug effects; Memory - physiology; Neuronal Plasticity - drug effects; Physical Conditioning, Animal - physiology; Rats; Rats, Wistar; Restraint, Physical - physiology; Restraint, Physical - psychology; Sexual Maturation - drug effects; Sexual Maturation - physiology; Social Environment; Spatial Learning - drug effects; Spirulina; Spirulina - chemistry; Stress, Psychological - complications; Stress, Psychological - metabolism; Stress, Psychological - pathology; Stress, Psychological - psychology
• ISSN: 0018-506X; 1095-6867
• DOI: 10.1016/j.yhbeh.2019.03.004

Chronic exposure to stress during adolescent period has been demonstrated to impair cognitive functions and the dendritic morphology of pyramidal neurons in the rat hippocampal CA3 area. The present study investigated the combined protective effects of Spirulina platensis (SP), a supplement made from blue-green algae with neuroprotective properties, voluntary exercise (EX) and environmental enrichment (EE) against cognitive deficits, alternations in hippocampal BDNF levels, and abnormal neuronal remodeling in adult female rats (PND 60) induced by exposure to chronic restraint stress during adolescent period (PND 30–40). Rats were exposed to restraint stress (2 h/day for 10 days, PND 30–40). Then, the animals were subjected to treatment with SP (200 mg/kg/day), EX, EE and the combined treatments (SP + EX, and SP + EE) between PND 41 and 55 of age. Following the interventions, spatial learning and memory, passive avoidance performance, hippocampal dendritic morphology and BDNF levels were assessed. Results showed that plasma corticosterone levels increased at PND 40 and remained elevated at PND 55 and 70 in the stressed rats. Stressed rats showed deficits in spatial learning and memory and passive avoidance performance, decreased BDNF levels in the hippocampus, and reduced apical dendritic length and branch points of the CA3 pyramidal neurons. These deficits were alleviated by the SP, EX and EE, and the combined treatments, which accompanied with a decline in serum corticosterone in stressed animals. Some treatments even enhanced cognitive functions, and BDNF levels and neuroanatomical remodeling in the hippocampus of non-stressed animals. Our findings provide important evidences that physical activity, exposure to EE, and the SP treatment during adolescent period can protect against adolescent stress induced behavioral, biochemical and neuroanatomical impairments in adulthood. •Adolescent stress induces cognitive and hippocampal BDNF in adulthood.•Adolescent stress causes dendritic retraction in CA3 pyramidal neurons in adulthood.•Spirulina platensis (SP) treatment, enriched environment (EE) and exercise ameliorated stress effects.•Combined treatments were more effective against stress effects on BDNF and dendritic morphology.•Using SP, EE and physical activity are efficient therapeutic ways against adolescent stress.