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The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial
Purpose The aim of the present study was to evaluate the effect of myo-Inositol administration on oocyte quality, fertilization rate and embryo quality in patients with PCOS during assisted reproductive technology (ART) cycles. Methods Fifty infertile PCOS patients were randomly designated in two gr...
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| Published in: | Archives of gynecology and obstetrics 2019-06, Vol.299 (6), p.1701-1707 |
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| container_title | Archives of gynecology and obstetrics |
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| creator | Akbari Sene, Azadeh Tabatabaie, Azam Nikniaz, Hossein Alizadeh, Ahad Sheibani, Kourosh Mortezapour Alisaraie, Mona Tabatabaie, Maryam Ashrafi, Mahnaz Amjadi, Fatemehsadat |
| description | Purpose
The aim of the present study was to evaluate the effect of myo-Inositol administration on oocyte quality, fertilization rate and embryo quality in patients with PCOS during assisted reproductive technology (ART) cycles.
Methods
Fifty infertile PCOS patients were randomly designated in two groups. In the study group, patients received daily doses of 4 g myo-Inositol combined with 400 mg folic acid and in the control group patients received only 400 mg folic acid from 1 month before starting the antagonist cycle until the day of ovum pick up. Oocyte and embryo qualities were assessed according to European Society of Human Reproduction and Embryology (ESHRE) guidelines. The gene expression of PGK1, RGS2 and CDC42 as a factor of oocyte quality in granulosa cells was analyzed using real-time RT-PCR. Levels of total antioxidant capacity (TAC) and reactive oxygen species (ROS) were evaluated by chemiluminescence assay in follicular fluid.
Results
The percentage of metaphase II oocyte, fertilization rate and embryo quality significantly improved in the study group (
p
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| doi_str_mv | 10.1007/s00404-019-05111-1 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2199189558</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2199189558</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-d8ba499ce974e4465ec039740d02952875ba6f432bf14c6cc9151c8b1fceccd03</originalsourceid><addsrcrecordid>eNp9kc9uFDEMxiMEotvCC3BAkbhwGYjnf7ihCgpSJS7lHGUdz26qTLJNMq2mL8erkbIFJA6cbOn7-bPlj7FXIN6BEMP7JEQr2kqArEQHABU8YRtom7oSA8BTthHyoRf9cMJOU7oWAupx7J-zk0ZIkKLpN-zH1Z74vIbK-pBsDo7TNBFmHjzPRQoB10z8ZtHO5pVrb_hEMVtn73W2BYq6yHoOfsfvwkye39m854fgVlxTtsjDrY4rT6s3seh88YbiLtjC65RsymR4pEMMZsFsb4lnwr0PLuxWjis6Sh-4Llu8CbO9LzA66y1qx3O02r1gzybtEr18rGfs--dPV-dfqstvF1_PP15W2Axdrsy41a2USHJoqW37jlA0pRdG1LKrx6Hb6n4qr9tO0GKPKKEDHLcwISEa0Zyxt0ffcunNQimr2SYk57SnsCRVg5Qwyq4bC_rmH_Q6LNGX61RddyP0TVsPhaqPFMaQUqRJHaKdy6sUCPUQrzrGq0q86le8CsrQ60frZTuT-TPyO88CNEcgFcnvKP7d_R_bnxWotbU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2258163427</pqid></control><display><type>article</type><title>The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial</title><source>Springer Nature</source><creator>Akbari Sene, Azadeh ; Tabatabaie, Azam ; Nikniaz, Hossein ; Alizadeh, Ahad ; Sheibani, Kourosh ; Mortezapour Alisaraie, Mona ; Tabatabaie, Maryam ; Ashrafi, Mahnaz ; Amjadi, Fatemehsadat</creator><creatorcontrib>Akbari Sene, Azadeh ; Tabatabaie, Azam ; Nikniaz, Hossein ; Alizadeh, Ahad ; Sheibani, Kourosh ; Mortezapour Alisaraie, Mona ; Tabatabaie, Maryam ; Ashrafi, Mahnaz ; Amjadi, Fatemehsadat</creatorcontrib><description>Purpose
The aim of the present study was to evaluate the effect of myo-Inositol administration on oocyte quality, fertilization rate and embryo quality in patients with PCOS during assisted reproductive technology (ART) cycles.
Methods
Fifty infertile PCOS patients were randomly designated in two groups. In the study group, patients received daily doses of 4 g myo-Inositol combined with 400 mg folic acid and in the control group patients received only 400 mg folic acid from 1 month before starting the antagonist cycle until the day of ovum pick up. Oocyte and embryo qualities were assessed according to European Society of Human Reproduction and Embryology (ESHRE) guidelines. The gene expression of PGK1, RGS2 and CDC42 as a factor of oocyte quality in granulosa cells was analyzed using real-time RT-PCR. Levels of total antioxidant capacity (TAC) and reactive oxygen species (ROS) were evaluated by chemiluminescence assay in follicular fluid.
Results
The percentage of metaphase II oocyte, fertilization rate and embryo quality significantly improved in the study group (
p
< 0.05), but the number of retrieved oocytes and follicle count were not statistically different between groups. Furthermore, the gene expression of PGK1, RGS2 and CDC42 was significantly higher in the study group (
p
< 0.05) but no differences were found between two groups in terms of TAC and ROS levels.
Conclusions
The present study findings suggest that myo-Inositol alters the gene expression in granulosa cells and improves oocyte and embryo quality among PCOS patients undergoing ART.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-019-05111-1</identifier><identifier>PMID: 30919036</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Clinical trials ; Double-Blind Method ; Endocrinology ; Female ; Fertility ; Fertilization in Vitro ; Folic Acid - pharmacology ; Folic Acid - therapeutic use ; Gene expression ; Gynecologic Endocrinology and Reproductive Medicine ; Gynecology ; Human Genetics ; Humans ; Infertility, Female - therapy ; Inositol - pharmacology ; Inositol - therapeutic use ; Medicine ; Medicine & Public Health ; Obstetrics/Perinatology/Midwifery ; Oocytes - drug effects ; Oocytes - pathology ; Polycystic ovary syndrome ; Polycystic Ovary Syndrome - drug therapy ; Polycystic Ovary Syndrome - pathology ; Quality ; Reactive oxygen species ; Reproductive Techniques, Assisted - standards ; Reproductive technologies</subject><ispartof>Archives of gynecology and obstetrics, 2019-06, Vol.299 (6), p.1701-1707</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Archives of Gynecology and Obstetrics is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-d8ba499ce974e4465ec039740d02952875ba6f432bf14c6cc9151c8b1fceccd03</citedby><cites>FETCH-LOGICAL-c375t-d8ba499ce974e4465ec039740d02952875ba6f432bf14c6cc9151c8b1fceccd03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30919036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akbari Sene, Azadeh</creatorcontrib><creatorcontrib>Tabatabaie, Azam</creatorcontrib><creatorcontrib>Nikniaz, Hossein</creatorcontrib><creatorcontrib>Alizadeh, Ahad</creatorcontrib><creatorcontrib>Sheibani, Kourosh</creatorcontrib><creatorcontrib>Mortezapour Alisaraie, Mona</creatorcontrib><creatorcontrib>Tabatabaie, Maryam</creatorcontrib><creatorcontrib>Ashrafi, Mahnaz</creatorcontrib><creatorcontrib>Amjadi, Fatemehsadat</creatorcontrib><title>The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
The aim of the present study was to evaluate the effect of myo-Inositol administration on oocyte quality, fertilization rate and embryo quality in patients with PCOS during assisted reproductive technology (ART) cycles.
Methods
Fifty infertile PCOS patients were randomly designated in two groups. In the study group, patients received daily doses of 4 g myo-Inositol combined with 400 mg folic acid and in the control group patients received only 400 mg folic acid from 1 month before starting the antagonist cycle until the day of ovum pick up. Oocyte and embryo qualities were assessed according to European Society of Human Reproduction and Embryology (ESHRE) guidelines. The gene expression of PGK1, RGS2 and CDC42 as a factor of oocyte quality in granulosa cells was analyzed using real-time RT-PCR. Levels of total antioxidant capacity (TAC) and reactive oxygen species (ROS) were evaluated by chemiluminescence assay in follicular fluid.
Results
The percentage of metaphase II oocyte, fertilization rate and embryo quality significantly improved in the study group (
p
< 0.05), but the number of retrieved oocytes and follicle count were not statistically different between groups. Furthermore, the gene expression of PGK1, RGS2 and CDC42 was significantly higher in the study group (
p
< 0.05) but no differences were found between two groups in terms of TAC and ROS levels.
Conclusions
The present study findings suggest that myo-Inositol alters the gene expression in granulosa cells and improves oocyte and embryo quality among PCOS patients undergoing ART.</description><subject>Adult</subject><subject>Clinical trials</subject><subject>Double-Blind Method</subject><subject>Endocrinology</subject><subject>Female</subject><subject>Fertility</subject><subject>Fertilization in Vitro</subject><subject>Folic Acid - pharmacology</subject><subject>Folic Acid - therapeutic use</subject><subject>Gene expression</subject><subject>Gynecologic Endocrinology and Reproductive Medicine</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Infertility, Female - therapy</subject><subject>Inositol - pharmacology</subject><subject>Inositol - therapeutic use</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - pathology</subject><subject>Polycystic ovary syndrome</subject><subject>Polycystic Ovary Syndrome - drug therapy</subject><subject>Polycystic Ovary Syndrome - pathology</subject><subject>Quality</subject><subject>Reactive oxygen species</subject><subject>Reproductive Techniques, Assisted - standards</subject><subject>Reproductive technologies</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kc9uFDEMxiMEotvCC3BAkbhwGYjnf7ihCgpSJS7lHGUdz26qTLJNMq2mL8erkbIFJA6cbOn7-bPlj7FXIN6BEMP7JEQr2kqArEQHABU8YRtom7oSA8BTthHyoRf9cMJOU7oWAupx7J-zk0ZIkKLpN-zH1Z74vIbK-pBsDo7TNBFmHjzPRQoB10z8ZtHO5pVrb_hEMVtn73W2BYq6yHoOfsfvwkye39m854fgVlxTtsjDrY4rT6s3seh88YbiLtjC65RsymR4pEMMZsFsb4lnwr0PLuxWjis6Sh-4Llu8CbO9LzA66y1qx3O02r1gzybtEr18rGfs--dPV-dfqstvF1_PP15W2Axdrsy41a2USHJoqW37jlA0pRdG1LKrx6Hb6n4qr9tO0GKPKKEDHLcwISEa0Zyxt0ffcunNQimr2SYk57SnsCRVg5Qwyq4bC_rmH_Q6LNGX61RddyP0TVsPhaqPFMaQUqRJHaKdy6sUCPUQrzrGq0q86le8CsrQ60frZTuT-TPyO88CNEcgFcnvKP7d_R_bnxWotbU</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Akbari Sene, Azadeh</creator><creator>Tabatabaie, Azam</creator><creator>Nikniaz, Hossein</creator><creator>Alizadeh, Ahad</creator><creator>Sheibani, Kourosh</creator><creator>Mortezapour Alisaraie, Mona</creator><creator>Tabatabaie, Maryam</creator><creator>Ashrafi, Mahnaz</creator><creator>Amjadi, Fatemehsadat</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20190601</creationdate><title>The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial</title><author>Akbari Sene, Azadeh ; Tabatabaie, Azam ; Nikniaz, Hossein ; Alizadeh, Ahad ; Sheibani, Kourosh ; Mortezapour Alisaraie, Mona ; Tabatabaie, Maryam ; Ashrafi, Mahnaz ; Amjadi, Fatemehsadat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-d8ba499ce974e4465ec039740d02952875ba6f432bf14c6cc9151c8b1fceccd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Clinical trials</topic><topic>Double-Blind Method</topic><topic>Endocrinology</topic><topic>Female</topic><topic>Fertility</topic><topic>Fertilization in Vitro</topic><topic>Folic Acid - pharmacology</topic><topic>Folic Acid - therapeutic use</topic><topic>Gene expression</topic><topic>Gynecologic Endocrinology and Reproductive Medicine</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Infertility, Female - therapy</topic><topic>Inositol - pharmacology</topic><topic>Inositol - therapeutic use</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - pathology</topic><topic>Polycystic ovary syndrome</topic><topic>Polycystic Ovary Syndrome - drug therapy</topic><topic>Polycystic Ovary Syndrome - pathology</topic><topic>Quality</topic><topic>Reactive oxygen species</topic><topic>Reproductive Techniques, Assisted - standards</topic><topic>Reproductive technologies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akbari Sene, Azadeh</creatorcontrib><creatorcontrib>Tabatabaie, Azam</creatorcontrib><creatorcontrib>Nikniaz, Hossein</creatorcontrib><creatorcontrib>Alizadeh, Ahad</creatorcontrib><creatorcontrib>Sheibani, Kourosh</creatorcontrib><creatorcontrib>Mortezapour Alisaraie, Mona</creatorcontrib><creatorcontrib>Tabatabaie, Maryam</creatorcontrib><creatorcontrib>Ashrafi, Mahnaz</creatorcontrib><creatorcontrib>Amjadi, Fatemehsadat</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akbari Sene, Azadeh</au><au>Tabatabaie, Azam</au><au>Nikniaz, Hossein</au><au>Alizadeh, Ahad</au><au>Sheibani, Kourosh</au><au>Mortezapour Alisaraie, Mona</au><au>Tabatabaie, Maryam</au><au>Ashrafi, Mahnaz</au><au>Amjadi, Fatemehsadat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>299</volume><issue>6</issue><spage>1701</spage><epage>1707</epage><pages>1701-1707</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
The aim of the present study was to evaluate the effect of myo-Inositol administration on oocyte quality, fertilization rate and embryo quality in patients with PCOS during assisted reproductive technology (ART) cycles.
Methods
Fifty infertile PCOS patients were randomly designated in two groups. In the study group, patients received daily doses of 4 g myo-Inositol combined with 400 mg folic acid and in the control group patients received only 400 mg folic acid from 1 month before starting the antagonist cycle until the day of ovum pick up. Oocyte and embryo qualities were assessed according to European Society of Human Reproduction and Embryology (ESHRE) guidelines. The gene expression of PGK1, RGS2 and CDC42 as a factor of oocyte quality in granulosa cells was analyzed using real-time RT-PCR. Levels of total antioxidant capacity (TAC) and reactive oxygen species (ROS) were evaluated by chemiluminescence assay in follicular fluid.
Results
The percentage of metaphase II oocyte, fertilization rate and embryo quality significantly improved in the study group (
p
< 0.05), but the number of retrieved oocytes and follicle count were not statistically different between groups. Furthermore, the gene expression of PGK1, RGS2 and CDC42 was significantly higher in the study group (
p
< 0.05) but no differences were found between two groups in terms of TAC and ROS levels.
Conclusions
The present study findings suggest that myo-Inositol alters the gene expression in granulosa cells and improves oocyte and embryo quality among PCOS patients undergoing ART.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30919036</pmid><doi>10.1007/s00404-019-05111-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0067 |
| ispartof | Archives of gynecology and obstetrics, 2019-06, Vol.299 (6), p.1701-1707 |
| issn | 0932-0067 1432-0711 |
| language | eng |
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| source | Springer Nature |
| subjects | Adult Clinical trials Double-Blind Method Endocrinology Female Fertility Fertilization in Vitro Folic Acid - pharmacology Folic Acid - therapeutic use Gene expression Gynecologic Endocrinology and Reproductive Medicine Gynecology Human Genetics Humans Infertility, Female - therapy Inositol - pharmacology Inositol - therapeutic use Medicine Medicine & Public Health Obstetrics/Perinatology/Midwifery Oocytes - drug effects Oocytes - pathology Polycystic ovary syndrome Polycystic Ovary Syndrome - drug therapy Polycystic Ovary Syndrome - pathology Quality Reactive oxygen species Reproductive Techniques, Assisted - standards Reproductive technologies |
| title | The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial |
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