Vascular endothelial growth factor/polylactide-polyethyleneglycol-polylactic acid copolymer/basic fibroblast growth factor mixed microcapsules in promoting angiogenic differentiation of rat bone marrow mesenchymal stem cells in vitro

• Published in: Zhongguo xiu fu chong jian wai ke za zhi 2019-02, Vol.33 (2), p.243
• Main Authors: Zhao, Shengli, Yin, Jie, Yu, Qinghe, Zhang, Guowei, Min, Shaoxiong
• Format: Article
• Language: Chinese
• Subjects: Animals; Bone Marrow Cells; Capsules; Cell Differentiation; Cells, Cultured; Fibroblast Growth Factor 2; Mesenchymal Stem Cells - physiology; Polyesters; Polyethylene Glycols; Rats; Vascular Endothelial Growth Factor A
• ISSN: 1002-1892
• DOI: 10.7507/1002-1892.201808099

To observe the effect of vascular endothelial growth factor/polylactide-polyethyleneglycol-polylactic acid copolymer/basic fibroblast growth factor (VEGF/PELA/bFGF) mixed microcapsules in promoting the angiogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) . The BMSCs were isolated by the method of whole bone marrow adherent, and sub-cultured. The passage 3 BMSCs were identified by Wright-Giemsa staining and flow cytometry, and used for subsequent experiments. VEGF/PELA/bFGF (group A), PELA/bFGF (group B), VEGF/PELA (group C), and PELA (group D) microcapsules were prepared. The biodegradable ability and cytotoxicity of PELA microcapsule were determined，and the slow-released ability of VEGF/PELA/bFGF mixed microcapsules was measured. The passage 3 BMSCs were co-cultured with the extracts of groups A, B, C, and D, separately. At 1, 3, 7, 14, and 20 days after being cultured, the morphological changes of induced BMSCs were recorded. At 21 days, the induced BMSCs were tested for DiI-labeled