Effects of chlorantraniliprole exposure on detoxification enzyme activities and detoxification-related gene expression in the fat body of the silkworm, Bombyx mori

• Published in: Ecotoxicology and environmental safety 2019-07, Vol.176, p.58-63
• Main Authors: Mao, Tingting, Li, Fanchi, Fang, Yilong, Wang, Hui, Chen, Jian, Li, Mengxue, Lu, Zhengting, Qu, Jianwei, Li, Jinxin, Hu, Jiahuan, Cheng, Xiaoyu, Ni, Min, Li, Bing
• Format: Article
• Language: English
• Subjects: Bombyx mori; Chlorantraniliprole; Detoxification; Fat body
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2019.03.074

Chlorantraniliprole (CAP) can induce excessive calcium release from muscle of insects, causing muscle paralysis until death, and its residues in farmland can cause poisoning in Bombyx mori (B. mori), resulting in the failure of cocooning. No reports have investigated the effects of CAP exposure on detoxification enzyme activities and detoxification-related gene expression in B. mori. In the present study, we treated mulberry leaves with CAP by the leaf-dipping method, and then B. mori larvae were continuously fed with the polluted mulberry leaves. Moreover, the detoxification enzyme activities and the expressions of detoxification-related genes in the fat body of B. mori were examined. The results showed that at 24 h after CAP exposure, the activities of P450 and GST enzymes were all significantly increased, with P450 enzymes responding fastest. CarE enzyme activity was up-regulated in 24 h, and then it was decreased compared with the control group. Furthermore, the expressions of the key genes in the PI3K/Akt/CncC signaling pathway (PI3K, PDK, Akt, CncC and Keap1) at the mRNA were significantly increased. Western blotting analysis revealed that Akt was inhibited at the protein level, resulting in decreased expression of Keap1 and increased expression of CncC. These results indicated that the PI3K/Akt/CncC signaling pathway in the fat body of B. mori responded to CAP exposure and regulated the expressions of downstream detoxification enzymes, thus enhancing the detoxifying capability of B. mori. [Display omitted] •Chlorantraniliprole causes Bombyx mori poisoning, reduced the body weight.•Chlorantraniliprole affected detoxifying enzymatic activity and transcription of detoxification-related genes.•Chlorantraniliprole increased the transcriptional levels and protein levels of CncC.•PI3K/Akt/CncC pathway mediates the regulation of metabolic detoxification of Chlorantraniliprole.