Genetic and chemical approaches for surface charge engineering of enzymes and their applicability in biocatalysis: A review

Surface charge engineering has received considerable interest from the scientific and industrial community in the last few decades. Although it was previously hypothesized that the surface charge–charge interactions were not a fundamental force to determine protein folding and stability, many studie...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2019-07, Vol.116 (7), p.1795-1812
Main Authors: Pedersen, Jacob Nedergaard, Zhou, Ye, Guo, Zheng, Pérez, Bianca
Format: Article
Language:English
Subjects:
activity
Aqueous solutions
Catalysis
chemical modification
Communities
Engineering
Enzymes
Genetic engineering
Ionic liquids
Organic chemistry
Organic solvents
Protein engineering
Protein folding
Protein structure
Proteins
Reviews
Solvents
stability
Surface charge
Surface stability
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Summary:Surface charge engineering has received considerable interest from the scientific and industrial community in the last few decades. Although it was previously hypothesized that the surface charge–charge interactions were not a fundamental force to determine protein folding and stability, many studies today show that surface charge plays a key role determining protein structure and activity. This review aims to (a) highlight the value of surface charged engineering of proteins to improve enzyme stability and activity in aqueous media and in the presence of ionic liquids (ILs) and organic solvents, (b) describe the existing approaches (genetic engineering or chemical modifications) for surface charged engineering, and (c) demonstrate the applicability of these surface charged enzymes in biocatalysis. The review provides a new foundation for the scientific and research community to exploit the surface engineering of protein concept for the development of new enzymes that are more active and stable in the presence of ILs and organic solvents, thereby offering new opportunities for industrial biocatalysis. Furthermore, this review is a useful tool for researchers to decide the best available technology to improve their enzyme system/process. Surface charge engineering of enzymes by genetic engineering or chemical modifications, is a valuable tool to improve to activity and stability of enzymes in aqueous media and in the presence of ionic liquids, and organic solvents. In this work, surface engineering of different industrially relevant enzymes is reviewed, highlighting the applicability of these surface charged enzymes in biocatalysis.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.26979