PR3-ANCA-associated vasculitis is associated with a specific motif in the peptide-binding cleft of HLA-DP molecules

• Published in: Rheumatology (Oxford, England) England), 2019-11, Vol.58 (11), p.1942-1949
• Main Authors: Gregersen, Jon Waarst, Erikstrup, Christian, Ivarsen, Per, Glerup, Rie, Krarup, Elizabeth, Keller, Kresten Krarup, Hansen, Ib Tønder, Møller, Bjarne Kuno
• Format: Article
• Language: English
• Subjects: Adult; Aged; Alleles; Amino Acid Motifs - genetics; Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis - genetics; Case-Control Studies; Denmark; European Continental Ancestry Group - genetics; Exons; Female; Genetic Predisposition to Disease - genetics; Genotype; HLA-DP Antigens - genetics; HLA-DRB1 Chains - genetics; Homozygote; Humans; Male; Middle Aged; Myeloblastin - genetics; Peroxidase - genetics; Registries; Retrospective Studies; Risk Factors
• ISSN: 1462-0324; 1462-0332
• DOI: 10.1093/rheumatology/kez111

Abstract Objectives This study aimed to characterize the association between HLA alleles and ANCA-associated vasculitis (AAV) in a genetically homogeneous population, and to analyse the contribution of specific HLA molecule amino acid sequences to the risk of AAV. Methods We included 187 Danish patients with AAV and 1070 healthy controls. All were HLA typed at two-field resolution. The association of HLA alleles to PR3- or MPO-AAV was analysed. The contribution of the dominant molecular motifs of the HLA-DPB1 molecule to the risk of AAV was investigated by association studies that included specific amino acid sequences of the hypervariable regions in exon 2. Results Ninety-four percent of patients with PR3-AAV were carriers of HLA-DPB1*04:01 while all patients with PR3-AAV were carriers of an HLA-DPB1*04 allele, and 85% were homozygous. This was significantly more than in the control group (P < 0.0001). The association was even stronger when HLA-DPB1*04:02 and -DPB1*23:01 were included. HLA-DPB1*04:01, -DPB1*04:02 and -DPB1*23:01 share amino acids in positions 8–9, 69, 76 and 84–87 within the hypervariable regions, but only positions 69 and 84–87 contributed significantly to the disease risk. HLA-DRB1*15 was associated with an increased risk of developing PR3-AAV, while HLA-DRB1*04, -DRB1*07 and -DQB1*03 were associated with a reduced risk of kidney involvement in PR3-AAV. MPO-AAV was only weakly associated with HLA class I alleles. Conclusion PR3-AAV is strongly associated with the HLA-DPB1 alleles HLA-DPB1*04:01, -DPB1*04:02 and -DPB1*23:01, which share amino acid sequences crucial for the peptide-binding groove.