Determining the Protein Stability of Alzheimer’s Disease Protein, Amyloid Precursor Protein

Determining protein thermal stability is integral in biomedical research. Here, with the use of two thermal stability assays, we show the melting temperature of amyloid precursor protein, an Alzheimer’s disease related protein. The average melting temperature for amyloid precursor protein of 55.9 °C...

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Bibliographic Details
Published in:The Protein Journal 2019-08, Vol.38 (4), p.419-424
Main Authors: Delport, Alexandré, Hewer, Raymond
Format: Article
Language:English
Subjects:
Alzheimer Disease - metabolism
Alzheimer's disease
Amyloid beta-protein
Amyloid beta-Protein Precursor - chemistry
Amyloid precursor protein
Animal Anatomy
Biochemistry
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Ethylenediaminetetraacetic acid
Experimental methods
Fluorimetry
Fluorometry
HEK293 Cells
Histology
Humans
Medical research
Medicine, Experimental
Melt temperature
Melting
Morphology
Organic Chemistry
Precursors
Protein binding
Protein Stability
Proteins
Temperature
Thermal stability
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Summary:Determining protein thermal stability is integral in biomedical research. Here, with the use of two thermal stability assays, we show the melting temperature of amyloid precursor protein, an Alzheimer’s disease related protein. The average melting temperature for amyloid precursor protein of 55.9 °C was derived from differential scanning fluorometry (55.1 ± 0.3 °C) and cellular thermal melt (56.7 ± 0.7 °C). These experimental methods have significant application for Alzheimer’s disease research including their use for amyloid precursor protein stability profiling and for the identification of additional binding partners to further elucidate novel protein functions.
ISSN:1572-3887
1573-4943
1875-8355
DOI:10.1007/s10930-019-09829-4