Interleukin gene polymorphisms in chronic periodontitis: A case-control study in the Indian population

• Published in: Archives of oral biology 2019-05, Vol.101, p.156-164
• Main Authors: Majumder, Poulami, Panda, Suraj K., Ghosh, Sujay, Dey, Subrata Kumar
• Format: Article
• Language: English
• Subjects: Case-Control Studies; Chronic periodontitis; Chronic Periodontitis - genetics; Cross validation consistency; Dentistry; Genetic Predisposition to Disease; Genotype; Humans; India; Interleukin; Interleukins - genetics; Polymorphism, Single Nucleotide; Polymorphisms; Statistical analysis
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2019.03.015

•IL1α-889C/T, IL1β (-31C/T,-511A/G,+3954C/T) are significantly associated to CP risk.•IL10(-819C/T,-592C/A) associated to CP that suppresses the anti-inflammatory action.•Logistic regression analysis shows significant link between oral habits and CP.•The best gene-gene interaction model is IL1β + 3954C/T X IL1β-31C/T with CV of 10/10. The aim of the present study was to investigate the possible association between seven Interleukin (IL) gene polymorphisms and their interaction with the chronic inflammatory oral disease, chronic periodontitis in Indian population. A total of 357 study subjects (157 with chronic periodontitis and 200 with healthy control) were genotyped for IL1A -889C/T (rs1800587), IL1B -31C/T (rs1143627), IL1B -511A/G (rs16944), IL1B + 3954C/T (rs1143634), IL2 −330 T/G (rs2069762), IL4 -33C/T (rs2070874), IL6 −597 G/A (rs1800797), IL8 -251A/T (rs4073), IL10 -819C/T (rs1800871), IL10 -592A/C (rs1800872) and IL13 -1111C/T (rs1800925). Statistical analysis was performed using the statistical software package SPSS v16. SNPassoc and Multifactor Dimensionality Reduction algorithm v3.0.2. We found that the statistically significant association of IL1A-889C/T (rs1800587), IL1B -31C/T (rs1143627), IL1B -511A/G (rs16944) and IL1B + 3954C/T (rs1143634) gene polymorphisms with increased susceptibility of chronic periodontitis. The best gene-gene interaction model was IL1B(-31C/T) X IL1B(+3954C/T) X IL10(-819C/T) with 10/10 cross validation consistency. The variant allele of IL1A, IL1B, IL10 and IL13 were seemed to be linked with chronic periodontitis increased susceptibility. The results of this study also indicate that epidemiological factors especially oral habits also play an important role in the development of chronic periodontitis. This study concludes IL1A -889C/T (rs1800587), IL1B -31C/T (rs1143627), IL1B -511A/G (rs16944), IL1B + 3954C/T (rs1143634), IL10 -819C/T (rs1800871), IL10 -592A/C (rs1800872) and IL13 -1111C/T (rs1800925) gene polymorphisms are significantly associated with chronic periodontitis. This work also infers that the best interactive model comprised of IL1B and IL10 polymorphisms cumulatively increase the risk of chronic periodontitis.