Thermodynamics of protein folding: methodology, data analysis and interpretation of data

Thermodynamics of protein folding refers to the stability measurements where structural changes of a given protein in the presence of a denaturing agent are monitored by spectroscopic or calorimetric techniques. In macroscopic point of view, protein stability represents the ratio of the population o...

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Bibliographic Details
Published in:European biophysics journal 2019-05, Vol.48 (4), p.305-316
Main Authors: Shirdel, Seyedeh Akram, Khalifeh, Khosrow
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cell Biology
Data Analysis
Data processing
Equilibrium conditions
Folding
Kinetics
Life Sciences
Membrane Biology
Nanotechnology
Neurobiology
Protein Folding
Protein Stability
Proteins
Proteins - chemistry
Structural integrity
Structural stability
Technical Primer
Temperature
Thermodynamics
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Summary:Thermodynamics of protein folding refers to the stability measurements where structural changes of a given protein in the presence of a denaturing agent are monitored by spectroscopic or calorimetric techniques. In macroscopic point of view, protein stability represents the ratio of the population of its unfolded state to that of folded one in equilibrium condition, while in microscopic point of view, the stability is actually a net value from a combination of favorable and unfavorable contributions that affect the structural integrity of a protein molecule. In this manuscript, the principles and methodological aspects of thermodynamic studies and methods of data analysis as well as interpretation of the results are presented. Graphical abstract
ISSN:0175-7571
1432-1017
DOI:10.1007/s00249-019-01362-7