RNA-Seq analysis revealed genes associated with UV-induced cell necrosis through MAPK/TNF-α pathways in human dermal fibroblast cells as an inducer of premature photoaging

Exposing the skin to solar UV radiation induces cascades of signaling pathways and biological alterations such as redox imbalance, suppression of antioxidant genes and programmed cell death. Therefore, the aim of this study was to use RNA-Seq to unravel the effects of UV radiation on Normal Human Ad...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2020-01, Vol.112 (1), p.484-493
Main Authors: Alafiatayo, Akinola Adekoya, Lai, Kok-Song, Ahmad, Syahida, Mahmood, Maziah, Shaharuddin, Noor Azmi
Format: Article
Language:English
Subjects:
Antioxidant
Fibroblast cells
Photoaging
RNA-Seq
ROS
UV radiation
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Summary:Exposing the skin to solar UV radiation induces cascades of signaling pathways and biological alterations such as redox imbalance, suppression of antioxidant genes and programmed cell death. Therefore, the aim of this study was to use RNA-Seq to unravel the effects of UV radiation on Normal Human Adult Fibroblast cells (NHDF). Cells were exposed to UV (20 mJ/cm2 for 3 mins) and incubated for 24 h. Total mRNA from the cells generated libraries of 72,080,648 and 40,750,939 raw reads from UV-treated and control cells respectively. Of the differentially expressed genes (DEGs) produced 2,007 were up-regulated and 2,791 were down-regulated (fold change ≥2, p 
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2019.03.011