Regulatory role of metallothionein-1/2 on development of sex differences in a high-fat diet-induced obesity

To evaluate the role of metallothionein (MT) in sex differences of obesity, we examined the effect of MT on regulation of lipid accumulation in female and male wild type (WT) and MT1/MT2-null (MT-KO) mice. Male and female WT and MT-KO mice fed standard diet (SD) or high-fat diet (HFD) for 35 weeks....

Full description

Saved in:
Bibliographic Details
Published in:Life sciences (1973) 2019-06, Vol.226, p.12-21
Main Authors: Kawakami, Takashige, Takasaki, Satoshi, Kadota, Yoshito, Fukuoka, Daiki, Sato, Masao, Suzuki, Shinya
Format: Article
Language:English
Subjects:
Accumulation
Adipocytes
Adipose tissue
Androgen
Body fat
Castration
Diet
Estrogen
Gender
Gender aspects
Gender differences
High fat diet
Lipids
Males
Metallothionein
Mice
Obesity
Rodents
Sex
Sex differences
Weight
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To evaluate the role of metallothionein (MT) in sex differences of obesity, we examined the effect of MT on regulation of lipid accumulation in female and male wild type (WT) and MT1/MT2-null (MT-KO) mice. Male and female WT and MT-KO mice fed standard diet (SD) or high-fat diet (HFD) for 35 weeks. Surgical castration in male mice was also performed to examine the effects of androgen on fat accumulation under HFD condition. The fat mass and size of adipocytes in white adipose tissue (WAT) was greater in adult MT-KO mice than in WT mice after 35 weeks of SD feeding without gender differences, suggesting a role of MT in limiting WAT development during normal growth in both sexes. In female mice fed HFD, weights of WAT and body were greater in MT-KO mice than in WT mice, indicating that MT had a preventive role against excess fat accumulation. In male mice fed HFD, WAT weight hardly increased in MT-KO mice compared to the increase in WT mice. Surgically castrated WT males fed HFD had lower WAT weight compared with sham-treated mice, although castrated MT-KO males fed HFD had greater increases in WAT weight compared with sham-treated mice and castrated WT males. These data suggest that MT could enhance the preventive action of estrogen against excess fat accumulation, on the contrary, MT augmented the ability of androgen to increase fat accumulation. MT may act to modify the susceptibility to obesity under sex hormones.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2019.04.012