Near‐Infrared Bioluminescence Imaging with a through‐Bond Energy Transfer Cassette

A coelenterazine (CTZ) analogue emitting near‐infrared (NIR) bioluminescence was synthesized for through‐bond energy transfer (TBET)‐based imaging modalities. The analogue, named Cy5‐CTZ, was prepared by conjugating cyanine‐5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is int...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2019-08, Vol.20 (15), p.1919-1923
Main Authors: Abe, Masahiro, Nishihara, Ryo, Ikeda, Yuma, Nakajima, Takahiro, Sato, Moritoshi, Iwasawa, Naoko, Nishiyama, Shigeru, Paulmurugan, Ramasamy, Citterio, Daniel, Kim, Sung Bae, Suzuki, Koji
Format: Article
Language:English
Subjects:
Acetylene
Bioluminescence
Bond energy
dyes/pigments
Energy transfer
Fluorescence
imaging agents
Infrared imaging
Substrates
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Summary:A coelenterazine (CTZ) analogue emitting near‐infrared (NIR) bioluminescence was synthesized for through‐bond energy transfer (TBET)‐based imaging modalities. The analogue, named Cy5‐CTZ, was prepared by conjugating cyanine‐5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR‐shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5‐CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells. Place your TBETs! An analogue of coelenterazine (CTZ) conjugated to cyanine‐5 (Cy5) dye, named Cy5‐CTZ, emits near‐infrared (NIR) bioluminescence through through‐bond energy transfer (TBET). Cy5‐CTZ thus provides multiplex imaging modality emitting both NIR bioluminescence and fluorescence.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201900149