Rapid Salmonella detection in foods by motility enrichment on a modified semi-solid rappaport-vassiliadis medium

Modification of Rappaport-Vassiliadis enrichment broth into a semisolid motility medium (MSRV) provided a sensitive means for detecting Salmonella in contaminated foods. The type of peptone, the concentration of magnesium chloride, the presence of novobiocin and the temperature of incubation were de...

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Bibliographic Details
Published in:Journal of food protection 1986-07, Vol.49 (7), p.510-514
Main Authors: DeSmedt, J, Bolderdijk, R.F, Rappold, H, Lautenschlaeger, D
Format: Article
Language:English
Subjects:
Biological and medical sciences
FOOD HYGIENE
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
GROWING MEDIA
HIGIENE DE LOS ALIMENTOS
HYGIENE DES ALIMENTS
MEDIO DE CULTIVO
MIGRACION
MIGRATION
MOUVEMENT
MOVEMENT
MOVIMIENTO
SALMONELLA
SUBSTRAT DE CULTURE
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Summary:Modification of Rappaport-Vassiliadis enrichment broth into a semisolid motility medium (MSRV) provided a sensitive means for detecting Salmonella in contaminated foods. The type of peptone, the concentration of magnesium chloride, the presence of novobiocin and the temperature of incubation were determinants in medium performance. The analytical procedure consists of preenrichment for 20 h, followed by motility enrichment on MSRV for 24 h and, if there is migration, serological tests with the motile culture. The test result is obtained within 48 h from the start of preenrichment. This approach gave 39% more Salmonella -positive samples than enrichment in tetrathionate brilliant green broth with subsequent plating.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-49.7.510