Panax ginseng methabolit (GIM-1) prevents oxidative stress and apoptosis in human Sertoli cells exposed to Monobutyl-phthalate (MBP)

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2019-06, Vol.86, p.68-75
• Main Authors: DE FREITAS, André Teves A.G., Figueiredo PINHO, Cristiane, de AQUINO, Ariana Musa, FERNANDES, Ana Angélica H., Fantin DOMENICONI, Raquel, JUSTULIN, Luis Antonio, SCARANO, Wellerson Rodrigo
• Format: Article
• Language: English
• Subjects: 8-Hydroxy-2'-Deoxyguanosine - metabolism; Apoptosis - drug effects; Caspase 3 - metabolism; Catalase - metabolism; Cell Line; Cytoprotection - drug effects; GIM-1; Glutathione Peroxidase - metabolism; Humans; Male; Monobutyl phthalate; NF-E2-Related Factor 2 - metabolism; Oxidative stress; Oxidative Stress - drug effects; Panax; Panax ginseng; Phthalic Acids - toxicity; Protective Agents - pharmacology; Sertoli cells; Sertoli Cells - drug effects; Sertoli Cells - metabolism; Sirtuin 1 - metabolism; Superoxide Dismutase - metabolism
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2019.02.008

•MBP increases apoptosis in HSec Sertoli cells.•MBP induces oxidative stress by suppressing antioxidant enzymes.•GIM-1 improves antioxidant mechanisms in HSec Sertoli cells.•GIM-1 is an important antagonist for MBP effects. This study evaluated oxidative stress markers in Human Sertoli cells cultivated on Geltrex® and exposed to Monobutyl Phthalate (MBP), and the potential cytoprotective role of GIM-1 on the antioxidant response. Exposure was performed at 30 min, 1, 12 and 48 h into 4 groups: control, MBP (10μM), GIM-1 (0,05μM) and MBP + GIM-1. Morphology was evaluated. Antioxidant enzymes were analyzed by colorimetric method; NRF-2, SIRT-1, 8- OHdG and Cleaved Caspase-3 by Western Blot. Larger spaces between cells were shown in MBP treatment; GIM-1 was similar to Control and MBP + GIM-1 showed an intermediate aspect. MBP reduced enzymatic activity of all enzymes and NRF-2 expression, increasing cleaved Caspase-3 expression; while GIM-1 increased antioxidants markers alone and attenuated MPB effects in MBP + GIM-1. MBP induced deleterious effects on Sertoli cells, increasing the oxidative stress, apoptosis and modifying their distribution in culture; however, GIM-1 acted as an important cytoprotective agent reversing our attenuating MBP effects.