Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway

To analyze the anti-tumor mechanism of Baicalin in human colon cancer. The MTT assay and colony formation assay demonstrated that Baicalin treatment inhibits the proliferation of DLD1 and HCT-116 cells. The apoptosis rates were induced upon Baicalin treatment and which was determined by FACS. The qP...

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Bibliographic Details
Published in:Molecular biology reports 2019-04, Vol.46 (2), p.1693-1700
Main Authors: Jia, Yanmei, Chen, Lirong, Guo, Songjia, Li, Yuanhong
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Apoptosis
Apoptosis - drug effects
Apoptosis - genetics
Baicalin
Biomedical and Life Sciences
c-Myc protein
Cell Line, Tumor - drug effects
Cell proliferation
Cell Proliferation - drug effects
Colon - metabolism
Colon cancer
Colonic Neoplasms - drug therapy
Colonic Neoplasms - genetics
Colonic Neoplasms - metabolism
Colorectal cancer
Dkk1 protein
Embryos
Flavonoids - metabolism
Flavonoids - pharmacology
Flow cytometry
HCT116 Cells
Histology
Humans
Intercellular Signaling Peptides and Proteins - genetics
Intercellular Signaling Peptides and Proteins - metabolism
Life Sciences
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Morphology
Myc protein
Original Article
Signal transduction
siRNA
Wnt protein
Wnt Signaling Pathway - drug effects
Wnt Signaling Pathway - genetics
β-catenin
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Summary:To analyze the anti-tumor mechanism of Baicalin in human colon cancer. The MTT assay and colony formation assay demonstrated that Baicalin treatment inhibits the proliferation of DLD1 and HCT-116 cells. The apoptosis rates were induced upon Baicalin treatment and which was determined by FACS. The qPCR and western blot analysis showed that Baicalin promotes expression of DKK1 (Dickkopf), an important antagonist of Wnt signaling pathway, thereby reduces the expression of its downstream protein β-catenin and c-Myc. Reduction of DKK1 expression by siRNA attenuates β-catenin and c-Myc expression. microRNA-217 (miR-217) is decreased upon Baicalin treatment. Moreover, DKK1 is identified as the direct downstream target gene of miR-217 through the dual-luciferase reporter assay. miR-217/DKK1-mediated inhibition of Wnt signaling pathway participate in apoptosis induced by Baicalin.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-019-04618-9