Germination and outgrowth of Bacillus subtilis spores in the presence of selected antioxidants

Germination and outgrowth of Bacillus subtilis spores was investigated using laboratory media containing butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tertiary butylhydroquinone (TBHQ) and propyl gallate (PG). Although all antioxidants inhibited or retarded germination initiation a...

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Bibliographic Details
Published in:Journal of food protection 1987-03, Vol.50 (3), p.206-211
Main Authors: Al-Khayat, M.A, Blank, G, Biliaderis, C
Format: Article
Language:English
Subjects:
ANTIOXIDANTES
ANTIOXIDANTS
ANTIOXYDANT
BACILLUS SUBTILIS
Biological and medical sciences
CRECIMIENTO
CROISSANCE
ESPORAS
Food additives
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
GERMINACION
GERMINATION
GROWING MEDIA
GROWTH
MEDIO DE CULTIVO
SPORE
SPORES
SUBSTRAT DE CULTURE
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Summary:Germination and outgrowth of Bacillus subtilis spores was investigated using laboratory media containing butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tertiary butylhydroquinone (TBHQ) and propyl gallate (PG). Although all antioxidants inhibited or retarded germination initiation and outgrowth, only BHA and TBHQ were effective at relatively low concentrations (150 and 100 ppm, respectively). Furthermore, BHA and TBHQ (150 ppm) were also shown to reduce spore growth by approximately 1 and 4 log within 72 and 6 h, respectively. The difference in the number of survivors between thermally (10 min at 80°C) and BHA (150 ppm)-treated germinated spores indicated that the antioxidant was effective against only a certain portion of the total heat-sensitive spore population. Sporostasis caused by BHA appeared reversible by the addition of Tween 80.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-50.3.206