Comparative Transcriptome Analysis of Celery Leaf Blades Identified an R2R3-MYB Transcription Factor that Regulates Apigenin Metabolism

• Published in: Journal of agricultural and food chemistry 2019-05, Vol.67 (18), p.5265-5277
• Main Authors: Yan, Jun, Yu, Li, He, Lizhoung, Zhu, Longying, Xu, Shuang, Wan, Yanhui, Wang, Hong, Wang, Ying, Zhu, Weimin
• Format: Article
• Language: English
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.9b01052

Apigenin has been proven to possess many pharmacological properties, but the mechanism of regulation of apigenin biosynthesis in plants remains unclear. Apigenin is the main flavonoid in celery and is mainly accumulated in the middle stage of leaf blade development. In this study, comparative transcriptomic analysis revealed a large number of structural genes and transcription factor genes that may be involved in the apigenin metabolic pathway. On the basis of the apigenin content in different celery accessions, an R2R3-MYB transcription factor gene, named AgMYB1, was isolated from the high apigenin celery accession C014. Bioinformatics analysis indicated that AgMYB1 may be involved in flavonoid metabolism. AgMYB1 expression showed a positive relation with the expression of the apigenin accumulation marker gene FNSI and with the apigenin content in different celery tissues. Moreover, overexpression and antisense expression of AgMYB1 in transgenic celery plants significantly increased and reduced the expression of apigenin biosynthetic genes and the apigenin content, respectively. These findings suggest that AgMYB1 is involved in positive regulation of apigenin metabolism in celery.