MicroRNA-related markers associated with corpus luteum tropism in buffalo (Bubalus bubalis)

• Published in: Genomics (San Diego, Calif.) Calif.), 2020-01, Vol.112 (1), p.108-113
• Main Authors: Jerome, A., Bhati, J., Mishra, D.C., Chaturvedi, K.K., Rao, A.R., Rai, A., Sikka, P., Singh, I.
• Format: Article
• Language: English
• Subjects: Buffalo; Corpus luteum; microRNA; Pregnancy
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2019.01.018

The study was undertaken to decipher the microRNA (miRNA) related markers associated with corpus luteum (CL) tropism in buffalo. The data obtained from deep sequencing of CL tissue from different physiological stages was mined in silico for the identification of miRNA-related markers (SSR & SNP). From the present study, 5 annotated and 176 unannotated miRNA were deduced while comparing with Bos taurus genome. In addition, 4 SSRs and 9 SNPs were deduced from the miRNA sequences. These SSRs were on the genes viz. Eukaryotic translation initiation factor 1-like, myocyte enhancer factor 2A, beta casein, T cell receptor gamma cluster 1. The SNP positions on genes viz. PYGO1 (Pygopus family PHD finger 1), LOC100337244 (Multidrug resistance-associated protein 4), FTH1 (Ferritin heavy chain 1), LOC788634 (BOLA class I histocompatibility antigen), PLXND1 (Plexin D1) and UBC (Ubiquitin C) show that these genes play critical role in CL tropism during estrous cycle in buffalo. •First study to decipher the microRNA related markers associated with corpus luteum tropism in buffalo.•We deduced 5 annotated and 176 unannotated miRNA while comparing with Bos taurus genome in addition to 4 SSRs and 9 SNPs from miRNA sequences.•Location of SSRs on Eukaryotic translation initiation factor 1-like, myocyte enhancer factor 2A, beta casein, T cell receptor gamma cluster 1 genes and SNPs on Pygopus family PHD finger 1, Ferritin heavy chain 1, Plexin D1 and Ubiquitin C genes.