Comparison of chain conformation properties of bio-active fucosylated chondroitin sulfates from two different sea cucumbers

• Published in: International journal of biological macromolecules 2019-07, Vol.133, p.44-50
• Main Authors: Mou, Jiaojiao, Zhuang, Didi, Li, Qiang, Song, Weiguo, Yang, Jie
• Format: Article
• Language: English
• Subjects: Chain conformation; Fucosylated chondroitin sulfate; Sea cucumber; Worm-like cylinder model
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.04.080

The present studies aimed to investigate the chain conformation of fucosylated chondroitin sulfates from two different sea cucumbers Apostichopus japonicus (fCS-Aj), and Holothuria mexicana (fCS-Hm). The chain conformations were determined by SEC-MALLS-Visc-RI, indicating that the chain conformations of fCS-Aj and fCS-Hm were random coil and extended linear chain structure in PBS, respectively. Our results suggested that the formation of linear chain of fCS were due to a synergistic effect of molecular weight, sulfate content and sulfation patterns. The worm-like cylinder model indicated that fCS-Aj possessed more flexible chains compared with fCS-Hm. fCS-Aj and fCS-Hm all exhibited as negatively charged polymers with good thermal stability. The present studies were important for understanding the structure-activities relationship of fCSs. •The fucosylated chondroitin sulfate was obtained from two different sea cucumbers.•Chain conformation was tested by HPSEC-MALLS-Visc-RI and worm-like cylinder model.•The electrical property and thermal stability were also investigated.