Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

• Published in: Journal of biophotonics 2019-09, Vol.12 (9), p.e201800304-n/a
• Main Authors: Duan, Peng, Li, Junyi, Yang, Weiyingxue, Li, Xiandong, Long, Manman, Feng, Xiaobing, Zhang, Yuge, Chen, Chunling, Morais, Camilo L.M., Martin, Francis L., Luo, Jianbin, Liu, Dameng, Xiong, Chengliang
• Format: Article
• Language: English
• Subjects: biochemical changes; Biochemistry; Biological properties; Cholesterol; Deoxyribonucleic acid; DNA; Fourier transforms; FTIR; Glycogen; Infrared spectroscopy; Lipids; Liver; Phospholipids; progression; pure foetal‐type hepatoblastoma; Raman; Raman spectra; Raman spectroscopy; Ribonucleic acid; RNA; Tissues
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.201800304

The biomolecular events resulting from the progression of hepatoblastoma remain to be elucidated. Fourier‐transform infrared (FTIR) and Raman spectroscopies are capable of noninvasively and accurately capturing the biochemical properties of biological tissue from its pathological status. Our aim was to probe critial biomolecular changes of liver accompanying the progression of pure foetal hepatoblastoma (PFH) by FTIR and Raman spectroscopies. Herein, biochemical alterations were both evident in the FTIR spectra (regions of 3100‐2800 cm−1 and 1800‐900 cm−1) and the Raman spectra (region of 1800‐400 cm−1) among normal, borderline and malignant liver tissues. Compared with normal tissues, the ratios of protein‐to‐lipid, α‐helix‐to‐β‐sheet, RNA‐to‐DNA, CH3 methyl‐to‐CH2 methylene, glucose‐to‐phospholipids, and unsaturated‐to‐saturated lipids intensities were significantly higher in malignant tissues, while the ratios of RNA‐to‐Amide II, DNA‐to‐Amide II, glycogen‐to‐cholesterol and Amide I‐to‐Amide II intensities were remarkably lower. These biochemical alterations in the transition from normal to malignant have profound implications not only for cyto‐pathological classification but also for molecular understanding of PFH progression. The successive changes of the spectral characteristics have been shown to be consistent with the development of PFH, indicating that FTIR and Raman spectroscopies are excellent tools to interrogate the biochemical features of different grades of PFH. We demonstrate that FTIR and Raman spectroscopies can characterise the biochemical profiles of different grades of pure foetal hepatoblastoma. Progressive changes in spectral characteristics are noted including ratios of protein‐to‐lipid, α‐helix‐to‐β‐sheet, RNA‐to‐DNA, CH3 methyl‐to‐CH2 methylene, glucose‐to‐phospholipids and unsaturated‐to‐saturated lipid intensities being higher for malignant tissues, with ratios of RNA‐to‐Amide II, DNA‐to‐Amide II, glycogen‐to‐cholesterol and Amide I‐to‐Amide II intensities being lower. This lends new insights into pathophysiological mechanisms underlying disease progression.