Lectins and ELLSA as powerful tools for glycoconjugate recognition analyses

Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins wer...

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Bibliographic Details
Published in:Glycoconjugate journal 2019-04, Vol.36 (2), p.175-183
Main Authors: Wu, Albert M., Liu, Jia Hau
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Glycoconjugates
Glycomics - methods
Humans
Lectins
Lectins - chemistry
Lectins - metabolism
Life Sciences
Mini-Review
Molecular Probe Techniques
Pathology
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - metabolism
Protein Binding
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Summary:Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-specificities of applied lectins, a new classification system was introduced. (iii) Applications of lectins for the detection and identification of N -glycan and/or Tn glycotope in glycoconjugates were intergraded. (iv) The polyvalency of the glycotopes in glycans was found to play a critical role in glycan–lectin recognition. This is an unexplored area of glycobiology and one of the most promising directions toward the coming glycoscience transformation.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-019-09865-3