Antifibrotic effects of Fraxetin on carbon tetrachloride-induced liver fibrosis by targeting NF-κB/IκBα, MAPKs and Bcl-2/Bax pathways

• Published in: Pharmacological reports 2019-06, Vol.71 (3), p.409-416
• Main Authors: Wu, Bin, Wang, Rong, Li, Shengnan, Wang, Yuanyuan, Song, Fuxing, Gu, Yanqiu, Yuan, Yongfang
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; bcl-2-Associated X Protein - metabolism; Carbon Tetrachloride - pharmacology; Coumarins - pharmacology; Drug Safety and Pharmacovigilance; Fraxetin; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Liver - drug effects; Liver - metabolism; Liver Cirrhosis - chemically induced; Liver Cirrhosis - drug therapy; Liver Cirrhosis - metabolism; Liver fibrosis; Male; MAPKs; Mitogen-Activated Protein Kinase 1 - metabolism; NF-kappa B - metabolism; NF-KappaB Inhibitor alpha - metabolism; NF-κB; Original Article; Oxidative Stress - drug effects; Pharmacotherapy; Pharmacy; Rats; Rats, Sprague-Dawley; Signal Transduction - drug effects
• ISSN: 1734-1140; 2299-5684
• DOI: 10.1016/j.pharep.2019.01.008

•Fraxetin possessed a therapeutic effect on CCl4-induced liver fibrosis in rats.•Fraxetin mitigated inflammation and hepatocytes apoptosis in fibrotic liver.•Fraxetin inhibited NF-κB/IκBα and MAPKs signaling pathway to against inflammation.•Fraxetin regulated Bcl-2/Bax signaling pathway to attenuate hepatocytes apoptosis. Liver fibrosis is a chronic lesion which ultimately results in cirrhosis and possible death. Although the high incidence and lethality, few therapies are effective for liver fibrosis. Fraxetin (7,8-dihydroxy-6-methoxy coumarin), a natural product extracted from cortex fraxini, has exhibited a significant hepatoprotective and anti-fibrotic properties. However, the underlying mechanism of the anti-hepatic fibrotic property remains unknown. 48 Male Sprague Dawley rats were divided into four groups at random which were named as normal group, model group, fraxetin 25 mg/kg and 50 mg/kg group. The experimental model of liver fibrosis was founded by carbon tetrachloride (CCl4) rats which were simultaneously treated with fraxetin (25 mg/kg or 50 mg/kg). Normal groups received equal volumes of saline and peanut oil. Results showed that fraxetin ameliorated CCl4 induced liver damage and fibrosis. Furthermore, histopathology examinations revealed that fraxetin improved the morphology and alleviated collagen deposition in fibrotic liver. Fraxetin inhibited inflammation and hepatocytes apoptosis by modulating the NF-κB/IκBα, MAPKs and Bcl-2/Bax signaling pathways. Our findings indicate that fraxetin is effective in preventing liver fibrosis through inhibiting inflammation and hepatocytes apoptosis which is associated with regulating NF-κB/IκBα, MAPKs and Bcl-2/Bax signaling pathways in rats.