Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

• Published in: Journal of cellular physiology 2019-11, Vol.234 (11), p.20742-20754
• Main Authors: Mohammadshirazi, Atiyeh, Sadrosadat, Hoda, Jaberi, Razieh, Zareikheirabadi, Masoomeh, Mirsadeghi, Sara, Naghdabadi, Zahra, Ghaneezabadi, Mahdieh, Fardmanesh, Mehdi, Baharvand, Hossein, Kiani, Sahar
• Format: Article
• Language: English
• Subjects: Animal behavior; Assessments; Cell differentiation; Cell proliferation; Cell survival; Chloride; contusion; Contusions; derived neural stem cells; Differentiation; hNSC; Injuries; Lithium; Lithium chloride; Locomotor activity; Motor evoked potentials; Nestin; Neural stem cells; Recovery; Recovery of function; Spinal cord injuries; spinal cord injury; Stem cell transplantation; Stem cells; Therapy; Transplantation
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.28680

A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and motor evoked potential (MEP) were performed to evaluate motor recovery as well as histological assessments to determine mechanisms of improvement. In accordance with our results, the hNSCs + Li and the Li groups showed significant improvement in locomotor scores and MEP. Also, Histological assessments revealed that transplanted hNSCs are capable of differentiation and migration along the spinal cord. Although NESTIN‐positive cells were proliferated significantly in the Lithium group in comparison with control and the hNSCs + Li groups, the quantity of ED1 cells in the hNSCs + Li was significantly larger than the other two groups. Our results demonstrate that combinational therapy of hNSCs with lithium chloride and lithium chloride individually are adequate for ameliorating more than partial functional recovery and endogenous repair in spinal cord‐injured rats.