Photoelectrochemically driven bioconversion and determination of nifedipine based on a double photoelectrode system

• Published in: Biosensors & bioelectronics 2019-06, Vol.135, p.160-165
• Main Authors: Tian, Jiuying, Huang, Mingjuan, Yang, Yanting, Wang, Dongyue, Lu, Jusheng
• Format: Article
• Language: English
• Subjects: Cytochrome P450; Double photoelectrode; Enzymatic bioconversion and determination; Nifedipine; Photoelectrochemically driven
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2019.04.020

In the present work, a double photoelectrode system has been constructed for photoelectrochemically driven enzymatic bioconversion and determination of nifedipine. In which, the TiO2 nanotube arrays in-situ assembled with g-C3N4 (TNA/g-C3N4) was used as a photoanode, and a cytochrome P450 3A4 (CYP3A4) enzyme was immobilized in the porous ITO/CuO films to fabricate an ITO/CuO/CYP3A4 photocathode. The constructed double photoelectrode system had a significant photocurrent response compared to the single ITO/CuO/CYP3A4 or TNA/g-C3N4 under visible light irradiation. Under optimal conditions, the photocurrent of the double photoelectrode system had a high catalytic activity toward substrate nifedipine with kcat of 5.62 s−1 and catalytic efficiency with kcat/kmapp of 0.94 μM−1 s−1, and the bioconversion yield of nifedipine reached 22.1%. Furthermore, the constructed double photoelectrode system could be used to determine the nifedipine concentration with a high sensitivity of 2.46 μA μM−1 and a low detection limit of 0.015 μM. Therefore, the proposed double photoelectrode system can be used well for study enzyme biocatalysis for target bioconversion, and also has a potential application for toxicity analysis. •A double photoelectrode system for enzymatic bioconversion and determination of nifedipine.•Improvement of PEC performance of TNAs based on the generation of g-C3N4 in-situ.•Good enzymatic property of CYP3A4 enzyme confined in porous CuO films.•Nifedipine determination with linear range of 0.05–100 μM and LOD of 0.015 μM.•High catalytic activity of the double photoelectrode system towards nifedipine bioconversion.